ArticleThe Journal of biological chemistry2011
Characterization of the activities of the CpeY, CpeZ, and CpeS bilin lyases in phycoerythrin biosynthesis in Fremyella diplosiphon strain UTEX 481.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 43 citations in OpenAlex.
- Partial photoswitching of rod-shaped phycobilisome production in the cyanobacterium Synechocystis sp. PCC 6803.Plant & cell physiology · 2025Article
- Characterization of Molecular Diversity and Organization of Phycobilisomes in Thermophilic Cyanobacteria.International journal of molecular sciences · 2023Article
- A hybrid type of chromatic acclimation regulated by the dual green/red photosensory systems in cyanobacteria.Plant physiology · 2022Article
- Phycobiliproteins-A Family of Algae-Derived Biliproteins: Productions, Characterization and Pharmaceutical Potentials.Marine drugs · 2022Review
- Crystal structure and molecular mechanism of an E/F type bilin lyase-isomerase.Structure (London, England : 1993) · 2022Article
- The phycoerythrobilin isomerization activity of MpeV inFrontiers in microbiology · 2022Article
- Molecular bases of an alternative dual-enzyme system for light color acclimation of marineProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Spectral and photochemical diversity of tandem cysteine cyanobacterial phytochromes.The Journal of biological chemistry · 2020Article
- Interplay between differentially expressed enzymes contributes to light color acclimation in marineProceedings of the National Academy of Sciences of the United States of America · 2019Article
- CpeF is the bilin lyase that ligates the doubly linked phycoerythrobilin on β-phycoerythrin in the cyanobacteriumThe Journal of biological chemistry · 2019Article
- Insights Into the Evolution of Picocyanobacteria and Phycoerythrin Genes (Frontiers in microbiology · 2019Article
- Adaptation to Blue Light in MarineFrontiers in microbiology · 2017Article
- Self-regulating genomic island encoding tandem regulators confers chromatic acclimation to marine Synechococcus.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Tryptophan-Rich Sensory Protein (TSPO) is Involved in Stress-Related and Light-Dependent Processes in the Cyanobacterium Fremyella diplosiphon.Frontiers in microbiology · 2015Article
- Insights into the biosynthesis and assembly of cryptophycean phycobiliproteins.The Journal of biological chemistry · 2014Article
- Structure and mechanism of the phycobiliprotein lyase CpcT.The Journal of biological chemistry · 2014Article
- Structural and biochemical characterization of the bilin lyase CpcS from Thermosynechococcus elongatus.Biochemistry · 2013Article
- A gene island with two possible configurations is involved in chromatic acclimation in marine Synechococcus.PloS one · 2013Article
- Phycoerythrin-specific bilin lyase-isomerase controls blue-green chromatic acclimation in marine Synechococcus.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- MpeV is a lyase isomerase that ligates a doubly linked phycourobilin on the β-subunit of phycoerythrin I and II in marine Synechococcus.The Journal of biological chemistryArticle
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7 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
When grown in green light, Fremyella diplosiphon strain UTEX 481 produces the red-colored protein phycoerythrin (PE) to maximize photosynthetic light harvesting. PE is composed of two subunits, CpeA and CpeB, which carry two and three phycoerythrobilin (PEB) chromophores, respectively, that are attached to specific Cys residues via thioether linkages. Specific bilin lyases are hypothesized to catalyze each PEB ligation. Using a heterologous, coexpression system in Escherichia coli, the PEB ligation activities of putative lyase subunits CpeY, CpeZ, and CpeS were tested on the CpeA and CpeB subunits from F. diplosiphon. Purified His(6)-tagged CpeA, obtained by coexpressing cpeA, cpeYZ, and the genes for PEB synthesis, had absorbance and fluorescence emission maxima at 566 and 574 nm, respectively. CpeY alone, but not CpeZ, could ligate PEB to CpeA, but the yield of CpeA-PEB was lower than achieved with CpeY and CpeZ together. Studies with site-specific variants of CpeA(C82S and C139S), together with mass spectrometric analysis of trypsin-digested CpeA-PEB, revealed that CpeY/CpeZ attached PEB at Cys(82) of CpeA. The CpeS bilin lyase ligated PEB at both Cys(82) and Cys(139) of CpeA but very inefficiently; the yield of PEB ligated at Cys(82) was much lower than observed with CpeY or CpeY/CpeZ. However, CpeS efficiently attached PEB to Cys(80) of CpeB but neither CpeY, CpeZ, nor CpeY/CpeZ could ligate PEB to CpeB.
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