ArticleBiomaterials2011
Engineering fibrin-binding TGF-β1 for sustained signaling and contractile function of MSC based vascular constructs.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Hydrogel Coating Optimization to Augment Engineered Soft Tissue Mechanics in Tissue-Engineered Blood Vessels.Bioengineering (Basel, Switzerland) · 2023Article
- Long-Term Controlled Growth Factor Release Using Layer-by-Layer Assembly for the Development ofACS applied materials & interfaces · 2022Article
- Stepwise Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells in Collagen Sponges under Different Microenvironments.International journal of molecular sciences · 2022Article
- Engineered Molecular Therapeutics Targeting Fibrin and the Coagulation System: a Biophysical Perspective.Biophysical reviews · 2022Review
- Cell-free vascular grafts that grow with the host.Advanced functional materials · 2020Article
- Engineering the mode of morphogenetic signal presentation to promote branching from salivary gland spheroids in 3D hydrogels.Acta biomaterialia · 2020Article
- Fibrin-based Bioinks: New Tricks from an Old Dog.International journal of bioprinting · 2020Review
- Adipose-derived stromal cell secreted factors induce the elastogenesis cascade within 3D aortic smooth muscle cell constructs.Matrix biology plus · 2019Article
- Functional Protein-Based Bioinspired Nanomaterials: From Coupled Proteins, Synthetic Approaches, Nanostructures to Applications.International journal of molecular sciences · 2019Review
- Influence of Cell Spreading Area on the Osteogenic Commitment and Phenotype Maintenance of Mesenchymal Stem Cells.Scientific reports · 2019Article
- Isolation and characterization ofExperimental and therapeutic medicine · 2017Article
- Vascular precursor cells in tissue injury repair.Translational research : the journal of laboratory and clinical medicine · 2017Review
- NANOG Restores Contractility of Mesenchymal Stem Cell-Based Senescent Microtissues.Tissue engineering. Part A · 2017Article
- Estrogen receptor β inhibits prostate cancer cell proliferation through downregulating TGF-β1/IGF-1 signaling.International journal of clinical and experimental pathology · 2017Article
- Laminin-111-derived peptide conjugated fibrin hydrogel restores salivary gland function.PloS one · 2017Article
- Hybrid Biomaterial with Conjugated Growth Factors and Mesenchymal Stem Cells for Ectopic Bone Formation.Tissue engineering. Part A · 2016Article
- Laminin-111 Peptides Conjugated to Fibrin Hydrogels Promote Formation of Lumen Containing Parotid Gland Cell Clusters.Biomacromolecules · 2016Article
- Hydrogel-Based Controlled Delivery Systems for Articular Cartilage Repair.BioMed research international · 2016Article
- Multiple-Step Injection Molding for Fibrin-Based Tissue-Engineered Heart Valves.Tissue engineering. Part C, Methods · 2015Article
- Capture of endothelial cells under flow using immobilized vascular endothelial growth factor.Biomaterials · 2015Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
We present a strategy to conjugate TGF-β1 into fibrin hydrogels to mimic the in vivo presentation of the growth factor in a 3D context. To this end, we engineered fusion proteins between TGF-β1 and a bi-functional peptide composed of a Factor XIII domain and a plasmin cleavage site. In another version the protease cleavage site was omitted to examine whether the growth factor that could not be released from the scaffold by cells had different effects on tissue constructs. The optimal insertion site which yielded correctly processed, functional protein was found between the latency associated peptide and mature TGF-β1 domains. In solution the fusion proteins exhibited similar biological activity as native TGF-β1 as evidenced by inhibition of cell proliferation and promoter activity assays. Immunoprecipitation experiments demonstrated that the fusion TGF-β1 protein bound to fibrinogen in a Factor XIII dependent manner and could be released from the peptide by the action of plasmin. In contrast to bolus delivery, immobilized TGF-β1 induced sustained signaling in fibrin-embedded cells for several days as evidenced by Smad2 phosphorylation. Prolonged pathway activation correlated with enhanced contractile function of vascular constructs prepared from hair follicle mesenchymal stem cells or bone marrow derived smooth muscle cells. Our results suggest that fibrin-immobilized TGF-β1 may be used to enhance the local microenvironment and improve the function of engineered tissues in vitro and potentially also after implantation in vivo where growth factor delivery faces overwhelming challenges.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.