ArticleInternational journal of pharmaceutics2012
Synthesis and characterization of mannosylated pegylated polyethylenimine as a carrier for siRNA.
Article in International journal of pharmaceutics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 69 citations in OpenAlex.
- Encapsulating Polyethyleneimine-DNA Nanoplexes into PEGylated Biodegradable Microparticles Increases Transgene Expression In Vitro and Reduces Inflammatory Responses In Vivo.AAPS PharmSciTech · 2021Article
- Modulation of Macrophages M1/M2 Polarization Using Carbohydrate-Functionalized Polymeric Nanoparticles.Polymers · 2020Review
- pH-sensitive zwitterionic polycarboxybetaine as a potential non-viral vector for small interfering RNA delivery.RSC advances · 2020Article
- Sustained Release of Hydrogen Sulfide (HAnnals of biomedical engineering · 2019Article
- Chemistry Routes for Copolymer Synthesis Containing PEG for Targeting, Imaging, and Drug Delivery Purposes.Pharmaceutics · 2019Review
- Oral delivery of siRNA lipid nanoparticles: Fate in the GI tract.Scientific reports · 2018Article
- Sustaining Intravitreal Residence With L-Arginine Peptide-Conjugated Nanocarriers.Investigative ophthalmology & visual science · 2017Article
- Peptide-Mediated Tumor Targeting by a Degradable Nano Gene Delivery Vector Based on Pluronic-Modified Polyethylenimine.Nanoscale research letters · 2016Article
- Designing polymers with sugar-based advantages for bioactive delivery applications.Journal of controlled release : official journal of the Controlled Release Society · 2015Review
- Combination of Nanoparticle-Delivered siRNA for Astrocyte Elevated Gene-1 (AEG-1) and All-trans Retinoic Acid (ATRA): An Effective Therapeutic Strategy for Hepatocellular Carcinoma (HCC).Bioconjugate chemistry · 2015Article
- Article
- Tuning PEGylation of mixed micelles to overcome intracellular and systemic siRNA delivery barriers.Biomaterials · 2015Article
- Towards Targeted Delivery Systems: Ligand Conjugation Strategies for mRNA Nanoparticle Tumor Vaccines.Journal of immunology research · 2015Review
- Design and evaluation of thioalkylated mannose-modified dendrimer (G3)/α-cyclodextrin conjugates as antigen-presenting cell-selective siRNA carriers.The AAPS journal · 2014Article
- DNA nanoparticles with core-shell morphology.Soft matter · 2014Article
- Enhanced localization of anticancer drug in tumor tissue using polyethylenimine-conjugated cationic liposomes.Nanoscale research letters · 2014Article
- Carbohydrate PEGylation, an approach to improve pharmacological potency.Beilstein journal of organic chemistry · 2014Review
- Mannosylated bioreducible nanoparticle-mediated macrophage-specific TNF-α RNA interference for IBD therapy.Biomaterials · 2013Article
- Recombinant high density lipoprotein nanoparticles for target-specific delivery of siRNA.Pharmaceutical research · 2013Article
- Mannosylated biodegradable polyethyleneimine for targeted DNA delivery to dendritic cells.International journal of nanomedicine · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Regulation of gene expression using small interfering RNA (siRNA) is a promising strategy for research and treatment of numerous diseases. In this study, we develop and characterize a delivery system for siRNA composed of polyethylenimine (PEI), polyethylene glycol (PEG), and mannose (Man). Cationic PEI complexes and compacts siRNA, PEG forms a hydrophilic layer outside of the polyplex for steric stabilization, and mannose serves as a cell binding ligand for macrophages. The PEI-PEG-mannose delivery system was constructed in two different ways. In the first approach, mannose and PEG chains are directly conjugated to the PEI backbone. In the second approach, mannose is conjugated to one end of the PEG chain and the other end of the PEG chain is conjugated to the PEI backbone. The PEI-PEG-mannose delivery systems were synthesized with 3.45-13.3 PEG chains and 4.7-3.0 mannose molecules per PEI. The PEI-PEG-Man-siRNA polyplexes displayed a coarse surface in Scanning Electron Microscopy (SEM) images. Polyplex sizes were found to range from 169 to 357 nm. Gel retardation assays showed that the PEI-PEG-mannose polymers are able to efficiently complex with siRNA at low N/P ratios. Confocal microscope images showed that the PEI-PEG-Man-siRNA polyplexes could enter cells and localized in the lysosomes at 2h post-incubation. Pegylation of the PEI reduced toxicity without any adverse reduction in knockdown efficiency relative to PEI alone. Mannosylation of the PEI-PEG could be carried out without any significant reduction in knockdown efficiency relative to PEI alone. Conjugating mannose to PEI via the PEG spacer generated superior toxicity and gene knockdown activity relative to conjugating mannose and PEG directly onto the PEI backbone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.