Evidence map›Paper›PMID 21863218›Full record

ArticleJournal of gastroenterology2011

Differential expression of miR-144* as a novel fecal-based diagnostic marker for colorectal cancer.

Murugan Kalimutho, Giovanna Del Vecchio Blanco, Serena Di Cecilia, Pierpaolo Sileri, Micaela Cretella, Francesco Pallone, Giorgio Federici, Sergio Bernardini

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In one paragraph

Article in Journal of gastroenterology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
66citing papers in PubMed, 3 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

66 citing papers in PubMed, 3 syntheses or guidelines pooled it.

  1. Pooled it
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  3. The expression and clinical significance of microRNAs in colorectal cancer detecting.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015
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  11. Novel Diagnostic Biomarkers in Colorectal Cancer.International journal of molecular sciences · 2022
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6 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Murugan KalimuthoDepartment of Internal Medicine, University of Rome "Tor Vergata", Via Montpellier 1, 00133 Rome, Italy. mceric_rugan@hotmail.com
Giovanna Del Vecchio Blanco
Serena Di Cecilia
Pierpaolo Sileri
Micaela Cretella
Francesco Pallone
Giorgio Federici
Sergio Bernardini

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMicroRNAs (miRNA) are tiny, noncoding, small, endogenous RNAs that play major roles in neoplastic transformation and could therefore offer a better quantitative and noninvasive method for the diagnosis and prognosis of colorectal cancer (CRC) using feces. In the present study, we screened feces for 648 miRNAs and analyzed the role of miR-144* as a potential CRC diagnostic marker.

methodsFecal miRNA expression was profiled with RT-pre-amplification-qPCR, and the stability was determined using both endogenous and exogenous miRNA by RT-qPCR. ROC analysis was performed to enhance the diagnosing power of the CRC patients' fecal specimens.

resultsWe detected 39% of all the miRNAs screened in feces. Endogenous miRNAs are more stable over time and temperature, while exogenous miRNAs degraded rapidly. miR-144* was overexpressed in feces, suggesting that it could be a potent candidate diagnostic marker for CRC detection, with a sensitivity of 74% and a specificity of 87% (n = 75, p < 0.0001). Moreover, RT-qPCR analysis showed that miR-144* was also overexpressed in paired CRC tissues, thus suggesting its possible utilization as a diagnostic marker.

conclusionsWe demonstrated that miRNAs are stable in the fecal microenvironment, and that, among them, miR-144* represents a novel fecal-based diagnostic marker for CRC screening. Nevertheless, our data need to be validated in a large cohort of subjects.

Indexed as

Gene Expression Regulation, NeoplasticAdultAgedAged, 80 and overBiomarkers, TumorColorectal NeoplasmsFecesFemaleHumansMaleMicroRNAsMiddle AgedReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityTemperatureTime FactorsBiomarkers, TumorMicroRNAsMIRN144 microRNA, human

Identifiers

PMID21863218

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.