ArticleThe Plant cell2011
Keep an eye on PPi: the vacuolar-type H+-pyrophosphatase regulates postgerminative development in Arabidopsis.
Article in The Plant cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
90 citing papers in PubMed, 164 citations in OpenAlex.
- Article
- Contrasting roles of reactive oxygen species in pyrophosphate-induced growth inhibition under normal and salt stress conditions.Plant molecular biology · 2025Article
- Retromer protein VPS29 plays a crucial and positive role in the sumoylation system mediated by E3 SUMO ligase SIZ1.The Plant journal : for cell and molecular biology · 2025Article
- UBIQUITIN-CONJUGATING ENZYME34 mediates pyrophosphatase AVP1 turnover and regulates abiotic stress responses in Arabidopsis.Plant physiology · 2025Article
- Regulated to respond: dual localization and dynamic control of AVP1 in plant carbon partitioning.Frontiers in plant science · 2025Review
- Adequate water supply enhances seedling growth and metabolism in Festuca kryloviana: insights from physiological and transcriptomic analys.BMC plant biology · 2024Article
- Arabidopsis COP1 guides stomatal response in guard cells through pH regulation.Communications biology · 2024Article
- Precocious cell differentiation occurs in proliferating cells in leaf primordia in ArabidopsisFrontiers in plant science · 2024Article
- Plasmodium falciparum utilizes pyrophosphate to fuel an essential proton pump in the ring stage and the transition to trophozoite stage.PLoS pathogens · 2023Article
- Proteomic exploration reveals a metabolic rerouting due to low oxygen during controlled germination of malting barley (Frontiers in plant science · 2023Article
- Editorial: Model organisms in plant science:Frontiers in plant science · 2023Article
- Mutations in type II Golgi-localized proton pyrophosphataseFrontiers in plant science · 2023Article
- Roles of type II HFrontiers in plant science · 2023Article
- Vacuolar H+-pyrophosphatase HVP10 enhances salt tolerance via promoting Na+ translocation into root vacuoles.Plant physiology · 2022Article
- Leaf-size control beyond transcription factors: Compensatory mechanisms.Frontiers in plant science · 2022Review
- Ultrasonic Waves Regulate Antioxidant Defense and Gluconeogenesis to Improve Germination From Naturally Aged Soybean Seeds.Frontiers in plant science · 2022Article
- Skotomorphogenesis exploits threonine to promote hypocotyl elongation.Quantitative plant biology · 2022Article
- Tonoplast proton pumps regulate nuclear spacing of female gametophytesFrontiers in plant science · 2022Article
- Tissue-targeted inorganic pyrophosphate hydrolysis in aFrontiers in plant science · 2022Article
- Proton-pumping pyrophosphatase homeolog expression is a dynamic trait in bread wheat (Plant direct · 2021Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Postgerminative growth of seed plants requires specialized metabolism, such as gluconeogenesis, to support heterotrophic growth of seedlings until the functional photosynthetic apparatus is established. Here, we show that the Arabidopsis thaliana fugu5 mutant, which we show to be defective in AVP1 (vacuolar H(+)-pyrophosphatase), failed to support heterotrophic growth after germination. We found that exogenous supplementation of Suc or the specific removal of the cytosolic pyrophosphate (PPi) by the heterologous expression of the cytosolic inorganic pyrophosphatase1 (IPP1) gene from budding yeast (Saccharomyces cerevisiae) rescued fugu5 phenotypes. Furthermore, compared with the wild-type and AVP1(Pro):IPP1 transgenic lines, hypocotyl elongation in the fugu5 mutant was severely compromised in the dark but recovered upon exogenous supply of Suc to the growth media. Measurements revealed that the peroxisomal β-oxidation activity, dry seed contents of storage lipids, and their mobilization were unaffected in fugu5. By contrast, fugu5 mutants contained ~2.5-fold higher PPi and ~50% less Suc than the wild type. Together, these results provide clear evidence that gluconeogenesis is inhibited due to the elevated levels of cytosolic PPi. This study demonstrates that the hydrolysis of cytosolic PPi, rather than vacuolar acidification, is the major function of AVP1/FUGU5 in planta. Plant cells optimize their metabolic function by eliminating PPi in the cytosol for efficient postembryonic heterotrophic growth.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.