ArticleVirology journal2011
Expression and characterization of UL16 gene from duck enteritis virus.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Duck enteritis virus UL21 is a late gene encoding a protein that interacts with pUL16.BMC veterinary research · 2020Article
- Duck Enteritis Virus VP16 Antagonizes IFN-Journal of immunology research · 2020Article
- US10 Protein Is Crucial but not Indispensable for Duck Enteritis Virus Infection in Vitro.Scientific reports · 2018Article
- Molecular characterization of duck enteritis virus UL41 protein.Virology journal · 2018Article
- RNA-seq comparative analysis of Peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus.Veterinary research · 2017Article
- Pathogenicity of duck plague and innate immune responses of the Cherry Valley ducks to duck plague virus.Scientific reports · 2016Article
- The transcription analysis of duck enteritis virus UL49.5 gene using real-time quantitative reverse transcription PCR.Virus genes · 2013Article
- Comparative genomic analysis of duck enteritis virus strains.Journal of virology · 2012Article
- Replication kinetics of duck enteritis virus UL16 gene in vitro.Virology journal · 2012Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrevious studies have indicated that the UL16 protein and its homologs from herpesvirus were conserved and played similar roles in viral DNA packaging, virion assembly, budding, and egress. However, there was no report on the UL16 gene product of duck enteritis virus (DEV). In this study, we analyzed the amino acid sequence of UL16 using bioinformatics tools and expressed in Escherichia coli Rosetta (DE3) induced by isopropy1-β-D-thiogalactopyranoside (IPTG). The recombinant protein was produced, purified using a Ni-NTA column and used to generate the polyclonal antibody against UL16. The intracellular distribution of the DEV UL16 product was carried out using indirect immunofluorescence assay.
resultsIn our study, UL16 gene of DEV was composed of 1089 nucleotides, which encoded 362 amino acids. Multiple sequence alignment suggested that the UL16 gene was highly conserved in herpesvirus family. The UL16 gene was cloned into a pET prokaryotic expression vector and transformed into Escherichia coli Rossetta (DE3) induced by IPTG. A 60kDa fusion protein band corresponding to the predicted size was produced on the SDS-PAGE, purified using a Ni-NTA column. Anti-UL16 polyclonal sera was prepared by immunizing rabbits, and reacted with a band in the IPTG induced cell lysates with an apparent molecular mass of 60 kDa. In vivo expression of the UL16 protein in DEV infected duck embryo fibroblast cells (DEFs) was localized mostly around perinuclear cytoplasmic area and in cytosol using indirect immunofluorescence assay.
conclusionsThe UL16 gene of DEV was successfully cloned, expressed and detected in DEV infected DEFs for the first time. The UL16 protein localized mostly around perinuclear cytoplasmic area and in cytosol in DEV infected DEFs. DEV UL16 shared high similarity with UL16 family members, indicating that DEV UL16 many has similar function with its homologs. All these results may provide some insight for further research about full characterizations and functions of the DEV UL16.
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