ArticleTissue engineering. Part A2012
Bone marrow-derived mesenchymal stromal cells enhance chimeric vessel development driven by endothelial cell-coated microtissues.
Article in Tissue engineering. Part A, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- High-Throughput Bioprinting of Geometrically-Controlled Pre-Vascularized Injectable Microgels for Accelerated Tissue Regeneration.Advanced healthcare materials · 2023Article
- Cellular infiltration in an injectable sulfated cellulose nanocrystal hydrogel and efficient angiogenesis by VEGF loading.Biomaterials research · 2023Article
- Bone marrow vasculature advancedFrontiers in cardiovascular medicine · 2023Review
- Bioengineering the Bone Marrow Vascular Niche.Frontiers in cell and developmental biology · 2021Review
- Article
- 3D biofabrication of vascular networks for tissue regeneration: A report on recent advances.Journal of pharmaceutical analysis · 2018Review
- Microfabrication of AngioChip, a biodegradable polymer scaffold with microfluidic vasculature.Nature protocols · 2018Article
- Mesenchymal Stromal/Stem Cells in Regenerative Medicine and Tissue Engineering.Stem cells international · 2018Review
- Modular tissue engineering for the vascularization of subcutaneously transplanted pancreatic islets.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Polyurethane Microgel Based Microtissue: Interface-Guided Assembly and Spreading.Langmuir : the ACS journal of surfaces and colloids · 2017Article
- Fate of modular cardiac tissue constructs in a syngeneic rat model.Journal of tissue engineering and regenerative medicine · 2015Article
- Platform technology for scalable assembly of instantaneously functional mosaic tissues.Science advances · 2015Article
- Spatial regulation of controlled bioactive factor delivery for bone tissue engineering.Advanced drug delivery reviews · 2015Review
- Vasculogenesis and Angiogenesis in Modular Collagen-Fibrin Microtissues.Biomaterials science · 2014Article
- Del-1 overexpression in endothelial cells increases vascular density in tissue-engineered implants containing endothelial cells and adipose-derived mesenchymal stromal cells.Tissue engineering. Part A · 2014Article
- Using Del-1 to tip the angiogenic balance in endothelial cells in modular constructs.Tissue engineering. Part A · 2014Article
- Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales.Acta biomaterialia · 2014Review
- Delivery of mesenchymal stem cells in chitosan/collagen microbeads for orthopedic tissue repair.Cells, tissues, organs · 2013Article
- Cotransplantation of adipose-derived mesenchymal stromal cells and endothelial cells in a modular construct drives vascularization in SCID/bg mice.Tissue engineering. Part A · 2012Article
- Toward an in vitro vasculature: differentiation of mesenchymal stromal cells within an endothelial cell-seeded modular construct in a microfluidic flow chamber.Tissue engineering. Part A · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Adding bone marrow-derived mesenchymal stromal cells (bmMSCs) to endothelialized collagen gel modules resulted in mature vessel formation, presumably caused in part by the observed display of pericyte-like behavior for the transplanted GFP(+) bmMSCs. A previous study determined that rat aortic endothelial cells (RAECs) delivered on the surface of small (∼0.8 mm long×0.5 mm diameter) collagen gel cylinders (microtissues, modular tissue engineering) formed vessels after transplantation into immunosuppressed Sprague-Dawley (SD) rats. Although the RAECs formed vessels in this allogeneic transplant model, there was a robust inflammatory response and the vessels that formed were leaky as shown by microcomputed tomography (microCT) perfusion studies. In vitro assays showed that SD rat bmMSCs embedded into the collagen gel modules increased the extent of EC proliferation and enhanced EC sprouting. In vivo, although vessel number was not affected, the new vessels formed by the bmMSCs and RAECs were more stable and leaked less in the microCT perfusion analysis than vessels formed by implanted RAECs alone. Addition of the bmMSCs also decreased the total number of CD68(+) macrophages that infiltrated the implant and changed the distribution of CD163(+) (M2) macrophages so that they were found within the newly developed vascularized tissue. Most interestingly, the bmMSCs became smooth muscle actin positive and migrated to surround the EC layer of the vessel, which is the location typical of pericytes. The combination of these two effects was presumed to be the cause of improved vascularity when bmMSCs were embedded in the EC-coated modules. Further exploration of these observations is warranted to exploit modular tissue engineering as a means of forming large vascularized functional tissues using microtissue components.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.