ArticleJournal of virology1990
Human immunodeficiency virus-like, nonreplicating, gag-env particles assemble in a recombinant vaccinia virus expression system.
Article in Journal of virology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 137 citations in OpenAlex.
- A multiclade env-gag VLP mRNA vaccine elicits tier-2 HIV-1-neutralizing antibodies and reduces the risk of heterologous SHIV infection in macaques.Nature medicine · 2021Article
- HIV-1-based Virus-like Particles that Morphologically Resemble Mature, Infectious HIV-1 Virions.Viruses · 2019Article
- Altering an artificial Gagpolnef polyprotein and mode of ENV co-administration affects the immunogenicity of a clade C HIV DNA vaccine.PloS one · 2012Article
- Article
- Phase III HIV vaccine trial in Thailand: a step toward a protective vaccine for HIV.Expert review of vaccines · 2010Review
- Bimodal AIDS vaccine approach: induction of cellular as well as humoral immunity can protect from systemic infection.Vaccine · 2010Article
- A replication-incompetent Rift Valley fever vaccine: chimeric virus-like particles protect mice and rats against lethal challenge.Virology · 2010Article
- The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus.Virology · 2009Article
- Pseudovirion particle production by live poxvirus human immunodeficiency virus vaccine vector enhances humoral and cellular immune responses.Journal of virology · 2005Article
- Multivesicular bodies as a platform for formation of the Marburg virus envelope.Journal of virology · 2004Article
- Incorporation of pol into human immunodeficiency virus type 1 Gag virus-like particles occurs independently of the upstream Gag domain in Gag-pol.Journal of virology · 2004Article
- Mucosal immunization with virus-like particles of simian immunodeficiency virus conjugated with cholera toxin subunit B.Journal of virology · 2003Article
- Rapid localization of Gag/GagPol complexes to detergent-resistant membrane during the assembly of human immunodeficiency virus type 1.Journal of virology · 2003Article
- Enhancement of mucosal immunization with virus-like particles of simian immunodeficiency virus.Journal of virology · 2003Article
- Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP.Journal of virology · 2002Article
- Role of RNA in facilitating Gag/Gag-Pol interaction.Journal of virology · 2002Article
- Role of immune responses against the envelope and the core antigens of simian immunodeficiency virus SIVmne in protection against homologous cloned and uncloned virus challenge in Macaques.Journal of virology · 1999Article
- Role of rubella virus glycoprotein domains in assembly of virus-like particles.Journal of virology · 1999Article
- Virus maturation by budding.Microbiology and molecular biology reviews : MMBR · 1998Review
- A proline-rich motif (PPPY) in the Gag polyprotein of Mason-Pfizer monkey virus plays a maturation-independent role in virion release.Journal of virology · 1998Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the assembly of human immunodeficiency virus (HIV)-like particles in African green monkey kidney cells coinfected with two recombinant vaccinia viruses, one carrying the HIV-1 gag and protease genes and the other the env gene. Biochemical analysis of particles sedimented from culture supernatants of doubly infected cells revealed that they were composed of gag proteins, primarily p24, as well as the env proteins gp120 and gp41. Thin-section immunoelectron microscopy showed that these particles were 100 to 120 nm in diameter, were characterized by the presence of cylindrical core structures, and displayed the mature gp120-gp41 complexes on their surfaces. Furthermore, thin-section immunoelectron microscopy analysis of infected cells showed that particle assembly and budding occurred at the plasma membrane. Nucleic acid hybridization suggested that the particles packaged only the gag mRNA but not the env mRNA. Therefore, the system we present is well suited for studies of HIV virion maturation. In addition, the HIV-like particles provide a novel and attractive approach for vaccine development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.