ArticleThe Journal of cell biology1990
In vivo analyses of integrin beta 1 subunit function in fibronectin matrix assembly.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 125 citations in OpenAlex.
- Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration.Frontiers in cell and developmental biology · 2019Article
- Plasma and cellular fibronectin: distinct and independent functions during tissue repair.Fibrogenesis & tissue repair · 2011Article
- Fibronectins, their fibrillogenesis, and in vivo functions.Cold Spring Harbor perspectives in biology · 2011Review
- PS integrins and laminins: key regulators of cell migration during Drosophila embryogenesis.PloS one · 2011Article
- PDGF-A interactions with fibronectin reveal a critical role for heparan sulfate in directed cell migration during Xenopus gastrulation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- PACSIN2 regulates cell adhesion during gastrulation in Xenopus laevis.Developmental biology · 2008Article
- Mystique is a new insulin-like growth factor-I-regulated PDZ-LIM domain protein that promotes cell attachment and migration and suppresses Anchorage-independent growth.Molecular biology of the cell · 2005Article
- The distribution and regulation of integrin-linked kinase in normal and diabetic kidneys.The American journal of pathology · 2001Article
- Decrease in fibronectin occurs coincident with the increased expression of its integrin receptor alpha5beta1 in stress-deprived ligaments.The Iowa orthopaedic journal · 1997Article
- Altered rate of fibronectin matrix assembly by deletion of the first type III repeats.The Journal of cell biology · 1996Article
- A novel role for the integrin-binding III-10 module in fibronectin matrix assembly.The Journal of cell biology · 1996Article
- Integrin beta 3 cytoplasmic tail is necessary and sufficient for regulation of alpha 5 beta 1 phagocytosis by alpha v beta 3 and integrin-associated protein.The Journal of cell biology · 1995Article
- Integrin alpha v beta 3 differentially regulates adhesive and phagocytic functions of the fibronectin receptor alpha 5 beta 1.The Journal of cell biology · 1994Article
- Single subunit chimeric integrins as mimics and inhibitors of endogenous integrin functions in receptor localization, cell spreading and migration, and matrix assembly.The Journal of cell biology · 1994Article
- Comparative evaluation of integrin alpha- and beta-chain expression in colorectal carcinoma cell lines and in their tumours of origin.Virchows Archiv : an international journal of pathology · 1994Article
- Functional role of the cytoplasmic domain of the integrin alpha 5 subunit.The Journal of cell biology · 1993Article
- Coordinated expression of extracellular matrix-degrading proteinases and their inhibitors regulates mammary epithelial function during involution.The Journal of cell biology · 1992Article
- Identification of the fibronectin sequences required for assembly of a fibrillar matrix.The Journal of cell biology · 1991Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early development of the urodele amphibian Pleurodeles waltl is accompanied by a process of progressive fibronectin (FN) fibrillogenesis. FN begins to assemble into fibrils on the inner surface of the blastocoele roof at the early blastula stage and progressively forms a complex extracellular matrix. We have analyzed the mechanisms of FN-fibril formation under normal and experimental conditions in vivo with the following probes: iodinated FN, fluorescein-labeled FN, synthetic peptides containing the Arg-Gly-Asp (RGD) cell surface recognition sequence of FN, and polyclonal antibodies against both beta 1 subunit of the amphibian FN receptor and the cytoplasmic domain of beta 1 subunit. We report that in living embryos, exogenous labeled mammalian FN injected into the amphibian blastocoele undergoes FN-fibril formation in spatiotemporal patterns similar to those of endogenous FN. This indicates regulation of fibrillogenesis by the cell surface rather than by changes in the type of FN. Fibrillogenesis is inhibited in a dose-dependent manner both by the GRGDS peptide and monospecific antibodies to amphibian integrin beta 1 subunit. Furthermore, when injected intracellularly into uncleaved embryos or into selected blastomeres, antibodies to the cytoplasmic domain of integrin beta 1 subunit produce a reversible inhibition of FN-fibril formation that follows early cell lineages and cause delays in development. Together, these data indicate that in vivo, the integrin beta 1 subunit and the RGD recognition signal are essential for the proper assembly of FN fibrils in early amphibian development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.