ArticleThe Journal of cell biology1990
Modulation of microtubule stability by kinetochores in vitro.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
- CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments.EMBO reports · 2024Article
- New insights into the mechanism for chromosome alignment in metaphase.International review of cell and molecular biology · 2013Review
- FORMIN a link between kinetochores and microtubule ends.Trends in cell biology · 2011Review
- An integrated mechanobiochemical feedback mechanism describes chromosome motility from prometaphase to anaphase in mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- A mechanobiochemical mechanism for monooriented chromosome oscillation in mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A bidirectional kinesin motor in live Drosophila embryos.Traffic (Copenhagen, Denmark) · 2005Article
- EB1 targets to kinetochores with attached, polymerizing microtubules.Molecular biology of the cell · 2002Article
- mini spindles: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila.The Journal of cell biology · 1999Article
- The dynamic behavior of individual microtubules associated with chromosomes in vitro.Molecular biology of the cell · 1998Article
- Dynamic elastic behavior of alpha-satellite DNA domains visualized in situ in living human cells.The Journal of cell biology · 1996Article
- Kinetochores moving away from their associated pole do not exert a significant pushing force on the chromosome.The Journal of cell biology · 1996Article
- Directional instability of kinetochore motility during chromosome congression and segregation in mitotic newt lung cells: a push-pull mechanism.The Journal of cell biology · 1993Article
- Role of GTP hydrolysis in microtubule dynamics: information from a slowly hydrolyzable analogue, GMPCPP.Molecular biology of the cell · 1992Article
- Microinjection of biotin-tubulin into anaphase cells induces transient elongation of kinetochore microtubules and reversal of chromosome-to-pole motion.The Journal of cell biology · 1992Article
- CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins.Chromosoma · 1991Article
- Chromosome motion during attachment to the vertebrate spindle: initial saltatory-like behavior of chromosomes and quantitative analysis of force production by nascent kinetochore fibers.The Journal of cell biology · 1991Article
- Identification of novel centromere/kinetochore-associated proteins using monoclonal antibodies generated against human mitotic chromosome scaffolds.The Journal of cell biology · 1991Article
- The Power of Xenopus Egg Extract for Reconstitution of Centromere and Kinetochore Function.Progress in molecular and subcellular biologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interface between kinetochores and microtubules in the mitotic spindle is known to be dynamic. Kinetochore microtubules can both polymerize and depolymerize, and their dynamic behavior is intimately related to chromosome movement. In this paper we investigate the influence of kinetochores on the inherent dynamic behavior of microtubules using an in vitro assay. The dynamics of microtubule plus ends attached to kinetochores are compared to those of free plus ends in the same solution. We show that microtubules attached to kinetochores exhibit the full range of dynamic instability behavior, but at altered transition rates. Surprisingly, we find that kinetochores increase the rate at which microtubule ends transit from growing to shrinking. This result contradicts our previous findings (Mitchison, T. J., and M. W. Kirschner, 1985b) for technical reasons which are discussed. We suggest that catalysis of the growing to shrinking transition by kinetochores may account for selective depolymerization of kinetochore microtubules during anaphase in vivo. We also investigate the effects of a nonhydrolyzable ATP analogue on kinetochore microtubule dynamics. We find that 5' adenylylimido diphosphate induces a rigor state at the kinetochore-microtubule interface, which prevents depolymerization of the microtubule.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.