ArticleCells, tissues, organs2012
Self-organization phenomena in embryonic stem cell-derived embryoid bodies: axis formation and breaking of symmetry during cardiomyogenesis.
Article in Cells, tissues, organs, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
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- Oviductal extracellular matrix hydrogels enhance in vitro culture of rabbit embryos and reduce deficiencies during assisted reproductive technologies.Scientific reports · 2024Article
- In Vitro Embryogenesis and Gastrulation Using Stem Cells in Mice and Humans.International journal of molecular sciences · 2023Review
- The Trophoblast Compartment Helps Maintain Embryonic Pluripotency and Delays Differentiation towards Cardiomyocytes.International journal of molecular sciences · 2023Article
- Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm.Protein & cell · 2023Article
- MLL3/MLL4 methyltransferase activities control early embryonic development and embryonic stem cell differentiation in a lineage-selective manner.Nature genetics · 2023Article
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- Development of Decellularized Oviductal Hydrogels as a Support for Rabbit Embryo Culture.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Multilayer and MATR3-dependent regulation of mRNAs maintains pluripotency in human induced pluripotent stem cells.iScience · 2021Article
- Article
- Cell Mechanics in Embryoid Bodies.Cells · 2020Review
- Bioengineered microenvironment to culture early embryos.Cell proliferation · 2020Review
- A Fiber Alginate Co-culture Platform for the Differentiation of mESC and Modeling of the Neural Tube.Frontiers in neuroscience · 2020Article
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- Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells.Nature communications · 2019Article
- Deconstructing and reconstructing the mouse and human early embryo.Nature cell biology · 2018Review
- Review
- Generation of organized germ layers from a single mouse embryonic stem cell.Nature communications · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Aggregation of embryonic stem cells gives rise to embryoid bodies (EBs) which undergo developmental processes reminiscent of early eutherian embryonic development. Development of the three germ layers suggests that gastrulation takes place. In vivo, gastrulation is a highly ordered process but in EBs only few data support the hypothesis that self-organization of differentiating cells leads to morphology, reminiscent of the early gastrula. Here we demonstrate that a timely implantation-like process is a prerequisite for the breaking of the radial symmetry of suspended EBs. Attached to a surface, EBs develop a bilateral symmetry and presumptive mesodermal cells emerge between the center of the EBs and a horseshoe-shaped ridge of cells. The development of an epithelial sheet of cells on one side of the EBs allows us to define an 'anterior' and a 'posterior' end of the EBs. In the mesodermal area, first cardiomyocytes (CMCs) develop mainly next to this epithelial sheet of cells. Development of twice as many CMCs at the 'left' side of the EBs breaks the bilateral symmetry and suggests that cardiomyogenesis reflects a local or temporal asymmetry in EBs. The asymmetric appearance of CMCs but not the development of mesoderm can be disturbed by ectopic expression of the muscle-specific protein Desmin. Later, the bilateral morphology becomes blurred by an apparently chaotic differentiation of many cell types. The absence of comparable structures in aggregates of cardiovascular progenitor cells isolated from the heart demonstrates that the self-organization of cells during a gastrulation-like process is a unique feature of embryonic stem cells.
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