ArticleMolecular and cellular biology2011
Negative elongation factor accelerates the rate at which heat shock genes are shut off by facilitating dissociation of heat shock factor.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- The Promoter-Proximal Pause: A Decision Point Governing RNA Polymerase II Fate.Annual review of biochemistry · 2026Review
- Evolution of promoter-proximal pausing enabled a new layer of transcription control.Nature structural & molecular biology · 2026Article
- A guide to heat shock factors as multifunctional transcriptional regulators.The FEBS journal · 2025Review
- Rapid P-TEFb-dependent transcriptional reorganization underpins the glioma adaptive response to radiotherapy.Nature communications · 2024Article
- The NELF pausing checkpoint mediates the functional divergence of Cdk9.Nature communications · 2023Article
- Article
- RNA polymerase II pausing in development: orchestrating transcription.Open biology · 2022Review
- Control of the activity of CAR-T cells within tumours via focused ultrasound.Nature biomedical engineering · 2021Article
- Motif 1 Binding Protein suppresses wingless to promote eye fate in Drosophila.Scientific reports · 2020Article
- NELF Regulates a Promoter-Proximal Step Distinct from RNA Pol II Pause-Release.Molecular cell · 2020Article
- Gene Expression in the Salivary Gland ofFrontiers in cellular and infection microbiology · 2019Article
- GFZF, a GlutathioneMolecular and cellular biology · 2018Article
- Rethinking HSF1 in Stress, Development, and Organismal Health.Trends in cell biology · 2017Review
- Identification of Regions in the Spt5 Subunit of DRB Sensitivity-inducing Factor (DSIF) That Are Involved in Promoter-proximal Pausing.The Journal of biological chemistry · 2017Article
- Article
- Molecular basis of HSF regulation.Nature structural & molecular biology · 2016Article
- RNA polymerase II pausing as a context-dependent reader of the genome.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2016Review
- RNA polymerase II pausing can be retained or acquired during activation of genes involved in the epithelial to mesenchymal transition.Nucleic acids research · 2015Article
- ATF1 modulates the heat shock response by regulating the stress-inducible heat shock factor 1 transcription complex.Molecular and cellular biology · 2015Article
- RNAi screen in Drosophila larvae identifies histone deacetylase 3 as a positive regulator of the hsp70 heat shock gene expression during heat shock.Biochimica et biophysica acta · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Promoter-proximal pausing of RNA polymerase II (Pol II) occurs on thousands of genes in animal cells. This pausing often correlates with the rapid induction of genes, but direct tests of the relationship between pausing and induction rates are lacking. hsp70 and hsp26 in Drosophila are rapidly induced by heat shock. Contrary to current expectations, depletion of negative elongation factor (NELF), a key factor in setting up paused Pol II, reduced pausing but did not interfere with rapid induction. Instead, depletion of NELF delayed the time taken for these genes to shut off during recovery from heat shock. NELF depletion also delayed the dissociation of HSF from hsp70 and hsp26, and a similar delay was observed when cells were depleted of the histone acetyltransferase CBP. CBP has been reported to associate with Pol II, and acetylation of HSF by CBP has been implicated in inhibiting the DNA-binding activity of HSF. We propose that NELF-mediated pausing allows Pol II to direct CBP-mediated acetylation of HSF, thus causing HSF to dissociate from the gene. Activators are typically viewed as controlling Pol II. Our results reveal a possible reciprocal relationship in which paused Pol II influences the activator.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.