ArticleNucleic acids research2011
Polynucleotide phosphorylase exonuclease and polymerase activities on single-stranded DNA ends are modulated by RecN, SsbA and RecA proteins.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Structural insights into RNA recognition by theProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Polynucleotide Phosphorylase Stops Replication Restart by Degrading the RNA within R-loops during Replication-Transcription Conflicts.bioRxiv : the preprint server for biology · 2026Article
- Pathological PNPase variants with altered RNA binding and degradation activity affect the phenotype of bacterial and human cell models.NAR molecular medicine · 2025Article
- RNase R vs. PNPase: selecting the best-suited exoribonuclease for environmental adaptation.Extremophiles : life under extreme conditions · 2024Review
- Processing of stalled replication forks in Bacillus subtilis.FEMS microbiology reviews · 2024Review
- Exoribonuclease RNase R protects AntarcticApplied and environmental microbiology · 2023Article
- Human PNPase causes RNA stabilization and accumulation of R-loops in the Escherichia coli model system.Scientific reports · 2023Article
- Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase.International journal of molecular sciences · 2022Review
- Pseudomonas aeruginosa Polynucleotide Phosphorylase Controls Tolerance to Aminoglycoside Antibiotics by Regulating the MexXY Multidrug Efflux Pump.Antimicrobial agents and chemotherapy · 2021Article
- Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage.Computational and structural biotechnology journal · 2021Review
- The minimal meningococcal ProQ protein has an intrinsic capacity for structure-based global RNA recognition.Nature communications · 2020Article
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- PNPase knockout results in mtDNA loss and an altered metabolic gene expression program.PloS one · 2018Article
- Pyrazinoic Acid Inhibits a Bifunctional Enzyme in Mycobacterium tuberculosis.Antimicrobial agents and chemotherapy · 2017Article
- Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli.BMC microbiology · 2017Article
- Molecular determinants for CRISPR RNA maturation in the Cas10-Csm complex and roles for non-Cas nucleases.Nucleic acids research · 2017Article
- Chromosomal transformation in Bacillus subtilis is a non-polar recombination reaction.Nucleic acids research · 2016Article
- Bacillus subtilis RecO and SsbA are crucial for RecA-mediated recombinational DNA repair.Nucleic acids research · 2015Article
- RNase III-Independent Autogenous Regulation of Escherichia coli Polynucleotide Phosphorylase via Translational Repression.Journal of bacteriology · 2015Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus subtilis pnpA gene product, polynucleotide phosphorylase (PNPase), is involved in double-strand break (DSB) repair via homologous recombination (HR) or non-homologous end-joining (NHEJ). RecN is among the first responders to localize at the DNA DSBs, with PNPase facilitating the formation of a discrete RecN focus per nucleoid. PNPase, which co-purifies with RecA and RecN, was able to degrade single-stranded (ss) DNA with a 3' → 5' polarity in the presence of Mn(2+) and low inorganic phosphate (Pi) concentration, or to extend a 3'-OH end in the presence dNDP · Mn(2+). Both PNPase activities were observed in evolutionarily distant bacteria (B. subtilis and Escherichia coli), suggesting conserved functions. The activity of PNPase was directed toward ssDNA degradation or polymerization by manipulating the Pi/dNDPs concentrations or the availability of RecA or RecN. In its dATP-bound form, RecN stimulates PNPase-mediated polymerization. ssDNA phosphorolysis catalyzed by PNPase is stimulated by RecA, but inhibited by SsbA. Our findings suggest that (i) the PNPase degradative and polymerizing activities might play a critical role in the transition from DSB sensing to end resection via HR and (ii) by blunting a 3'-tailed duplex DNA, in the absence of HR, B. subtilis PNPase might also contribute to repair via NHEJ.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.