ArticleJournal of biomolecular screening2011
A biochemical screen for identification of small-molecule regulators of the Wnt pathway using Xenopus egg extracts.
Article in Journal of biomolecular screening, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Feedback in the β-catenin destruction complex imparts bistability and cellular memory.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Chemical Screening Using Cell-FreeCold Spring Harbor protocols · 2018Article
- Pharmacologic Inhibition of β-Catenin With Pyrvinium Inhibits Murine and Human Models of Wilms Tumor.Oncology research · 2017Article
- Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Exploiting translational stalling peptides in an effort to extend azithromycin interaction within the prokaryotic ribosome nascent peptide exit tunnel.Bioorganic & medicinal chemistry · 2015Article
- Rapid synthesis, RNA binding, and antibacterial screening of a peptidic-aminosugar (PA) library.ACS chemical biology · 2015Article
- Small-molecule high-throughput screening utilizing Xenopus egg extract.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Macrolide-peptide conjugates as probes of the path of travel of the nascent peptides through the ribosome.ACS chemical biology · 2014Article
- Reconstitution Of β-catenin degradation in Xenopus egg extract.Journal of visualized experiments : JoVE · 2014Article
- Screening for small molecule inhibitors of embryonic pathways: sometimes you gotta crack a few eggs.Bioorganic & medicinal chemistry · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
19 authors.
Funding
Abstract
Misregulation of the Wnt pathway has been shown to be responsible for a variety of human diseases, most notably cancers. Screens for inhibitors of this pathway have been performed almost exclusively using cultured mammalian cells or with purified proteins. We have previously developed a biochemical assay using Xenopus egg extracts to recapitulate key cytoplasmic events in the Wnt pathway. Using this biochemical system, we show that a recombinant form of the Wnt coreceptor, LRP6, regulates the stability of two key components of the Wnt pathway (β-catenin and Axin) in opposing fashion. We have now fused β-catenin and Axin to firefly and Renilla luciferase, respectively, and demonstrate that the fusion proteins behave similarly as their wild-type counterparts. Using this dual luciferase readout, we adapted the Xenopus extracts system for high-throughput screening. Results from these screens demonstrate signal distribution curves that reflect the complexity of the library screened. Of several compounds identified as cytoplasmic modulators of the Wnt pathway, one was further validated as a bona fide inhibitor of the Wnt pathway in cultured mammalian cells and Xenopus embryos. We show that other embryonic pathways may be amendable to screening for inhibitors/modulators in Xenopus egg extracts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.