ArticleJournal of biomedical science2011
BMP-2 gene-fibronectin-apatite composite layer enhances bone formation.
Article in Journal of biomedical science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Synergistic effects of erythropoietin and selenium enhance bone regeneration in ovariectomized rats.Journal of materials science. Materials in medicine · 2026Article
- Tauroursodeoxycholic acid combined with selenium accelerates bone regeneration in ovariectomized rats.Journal of materials science. Materials in medicine · 2024Article
- Effects of the combination of bone morphogenetic protein-2 and nano-hydroxyapatite on the osseointegration of dental implants.Journal of the Korean Association of Oral and Maxillofacial Surgeons · 2021Article
- BMP-2 Gene Delivery-Based Bone Regeneration in Dentistry.Pharmaceutics · 2019Review
- When 1+1>2: Nanostructured composites for hard tissue engineering applications.Materials science & engineering. C, Materials for biological applications · 2015Review
- Area-specific cell stimulation via surface-mediated gene transfer using apatite-based composite layers.International journal of molecular sciences · 2015Article
- Nanostructured platforms for the sustained and local delivery of antibiotics in the treatment of osteomyelitis.Critical reviews in therapeutic drug carrier systems · 2015Review
- Mesenchymal stem cells overexpressing Ihh promote bone repair.Journal of orthopaedic surgery and research · 2014Article
- Sonoporation increases therapeutic efficacy of inducible and constitutive BMP2/7 in vivo gene delivery.Human gene therapy methods · 2014Article
- Dual delivery of BMP-2 and bFGF from a new nano-composite scaffold, loaded with vascular stents for large-size mandibular defect regeneration.International journal of molecular sciences · 2013Article
- Fabrication of DNA-antibody-apatite composite layers for cell-targeted gene transfer.Science and technology of advanced materials · 2012Article
- Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer.Journal of materials science. Materials in medicine · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSafe and efficient gene transfer systems are needed for tissue engineering. We have developed an apatite composite layer including the bone morphogenetic protein-2 (BMP-2) gene and fibronectin (FB), and we evaluated its ability to induce bone formation.
methodsAn apatite composite layer was evaluated to determine the efficiency of gene transfer to cells cultured on it. Cells were cultured on a composite layer including the BMP-2 gene and FB, and BMP-2 gene expression, BMP-2 protein concentrations, alkaline phosphatase (ALP) activity, and osteocalcin (OC) concentrations were measured. A bone defect on the cranium of rats was treated with hydroxyapatite (HAP)-coated ceramic buttons with the apatite composite layer including the BMP-2 gene and FB (HAP-BMP-FB). The tissue concentration of BMP-2, bone formation, and the expression levels of the BMP-2, ALP, and OC genes were all quantified.
resultsThe apatite composite layer provided more efficient gene transfer for the cultured cells than an apatite composite layer without FB. The BMP-2 concentration was approximately 100-600 pg/mL in the cell-culture medium. Culturing the cells on the apatite composite layer for 27 days increased ALP activity and OC concentrations. In animal experiments, the tissue concentrations of BMP-2 were over 100 pg/mg in the HAP-BMP-FB group and approximately 50 pg/mg in the control groups. Eight weeks later, bone formation was more enhanced in the HAP-BMP-FB group than in the control groups. In the tissues surrounding the HAP button, the gene expression levels of ALP and OC increased.
conclusionThe BMP-2 gene-FB-apatite composite layer might be useful for bone engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.