ArticleBiochemistry2011
Structure-guided engineering of a Pacific Blue fluorophore ligase for specific protein imaging in living cells.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Synthesis of Monofluorinated 7-Hydroxycoumarin-3-Carboxamides as Cell-Permeable Fluorescent Molecular Probes.ACS medicinal chemistry letters · 2024Article
- Further assessments of ligase LplA-mediated modifications of proteins in vitro and in cellulo.Molecular biology reports · 2022Article
- Imaging and targeting LOX-mediated tissue remodeling with a reactive collagen peptide.Nature chemical biology · 2021Article
- Site-specific conjugation of native antibody.Antibody therapeutics · 2020Article
- Implementing VIPER for Imaging Cellular Proteins by Fluorescence Microscopy.Bio-protocol · 2019Article
- Fluorescent Labeling of Protein Using Blue-Emitting 8-Amino-BODIPY Derivatives.Journal of fluorescence · 2017Article
- Versatile Interacting Peptide (VIP) Tags for Labeling Proteins with Bright Chemical Reporters.Chembiochem : a European journal of chemical biology · 2017Article
- A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging.Annual review of physiology · 2017Review
- Quantification of Small Molecule-Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore.ACS omega · 2016Article
- Site-Specific Radiofluorination of Biomolecules with 8-[(18)F]-Fluorooctanoic Acid Catalyzed by Lipoic Acid Ligase.ACS chemical biology · 2016Article
- Multiexcitation Fluorogenic Labeling of Surface, Intracellular, and Total Protein Pools in Living Cells.Bioconjugate chemistry · 2016Article
- Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases.Proteomes · 2015Review
- Computational design of a red fluorophore ligase for site-specific protein labeling in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Site-specific functionalization of proteins and their applications to therapeutic antibodies.Computational and structural biotechnology journal · 2014Review
- Enzymatic labeling of proteins: techniques and approaches.Bioconjugate chemistry · 2013Review
- Site-specific protein labeling using PRIME and chelation-assisted click chemistry.Nature protocols · 2013Article
- Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.ACS nano · 2012Article
- Site-specific protein modification using lipoic acid ligase and bis-aryl hydrazone formation.Chembiochem : a European journal of chemical biology · 2012Article
- Fluorophore targeting to cellular proteins via enzyme-mediated azide ligation and strain-promoted cycloaddition.Journal of the American Chemical Society · 2012Article
- Diels-Alder cycloaddition for fluorophore targeting to specific proteins inside living cells.Journal of the American Chemical Society · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mutation of a gatekeeper residue, tryptophan 37, in E. coli lipoic acid ligase (LplA), expands substrate specificity such that unnatural probes much larger than lipoic acid can be recognized. This approach, however, has not been successful for anionic substrates. An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, (E20G/W37T)LplA, that ligates Pacific Blue as efficiently as (W37V)LplA ligates 7-hydroxycoumarin. The utility of this Pacific Blue ligase for specific labeling of recombinant proteins inside living cells, on the cell surface, and inside acidic endosomes is demonstrated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.