ArticleCytotechnology2011
Optimal time for passaging neurospheres based on primary neural stem cell cultures.
Article in Cytotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Evidence for a Neural Stem Cell Niche in the Postnatal Rat Vestibular Ganglion: An In Vitro Study.Stem cells international · 2026Article
- Neurons in a Dish: A Review of In Vitro Cell Models for Studying Neurogenesis.Journal of neurochemistry · 2026Review
- Protocol for culturing neurospheres from progenitor cells in the dentate gyrus of aged mouse hippocampus.STAR protocols · 2025Article
- Assembling Spheroids of Rat Primary Neurons Using a Stress-Free 3D Culture System.International journal of molecular sciences · 2023Article
- Genome-wide pooled CRISPR screening in neurospheres.Nature protocols · 2023Review
- Characterization of neural stem cells derived from human stem cells from the apical papilla undergoing three-dimensional neurosphere induction.Journal of applied oral science : revista FOB · 2023Article
- Article
- Phosphatidylcholine restores neuronal plasticity of neural stem cells under inflammatory stress.Scientific reports · 2021Article
- Discovering the Potential of Dental Pulp Stem Cells for Corneal Endothelial Cell Production: A Proof of Concept.Frontiers in bioengineering and biotechnology · 2021Article
- Optimal culture conditions for neurosphere formation and neuronal differentiation from human dental pulp stem cells.Journal of applied oral science : revista FOB · 2021Article
- Guanosine Promotes Proliferation in Neural Stem Cells from Hippocampus and Neurogenesis in Adult Mice.Molecular neurobiology · 2020Article
- An Efficient Method for Generating Murine Hypothalamic Neurospheres for the Study of Regional Neural Progenitor Biology.Endocrinology · 2020Article
- Article
- Cost-Effective Cosmetic-Grade Hyaluronan Hydrogels for ReNcell VM Human Neural Stem Cell Culture.Biomolecules · 2019Article
- Administration of growth factors promotes salisphere formation from irradiated parotid salivary glands.PloS one · 2018Article
- Mechano growth factor, a splice variant of IGF-1, promotes neurogenesis in the aging mouse brain.Molecular brain · 2017Article
- Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone.Journal of visualized experiments : JoVE · 2016Article
- Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation.Stem cells international · 2016Article
- Isolation of Human Neural Stem Cells from the Amniotic Fluid with Diagnosed Neural Tube Defects.Stem cells and development · 2015Article
- Neurogenic maturation of human dental pulp stem cells following neurosphere generation induces morphological and electrophysiological characteristics of functional neurons.Stem cells and development · 2015Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cultured neural stem cells (NSCs) provide a powerful means for investigating central nervous system disease, neuron development, differentiation, and regeneration. To obtain sufficient neurospheres, subculturing is essential following establishment of the primary NSC culture. Passaging the primary neurospheres is a key issue that is often ignored. We evaluated the influence of different passaging schedules on primary cultured NSCs. Passaging was performed on day 5, 7 or 9. We observed more neurospheres with diameters of 200-250 μm on day 7 than on day 5 or 9. Prolonging the time of primary culture reduced the cell metabolic activity by the MTT assay and cell proliferation by colony-forming assay and the differentiation to neurons from cells at P2 and later decreased. Additionally, more cells were in G0/G1 phase, and higher expression of p16 ( INK4a ) and lower expression of cyclin D1 was found when the time of primary culture was prolonged to 9 days compared to 7-days cultures. Thus, in this study, we established that the optimal time for subculturing aggregated NSCs was on day 7 based on the primary culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.