ArticleMycopathologia2012
A model to evaluate the cytotoxicity of the fungal volatile organic compound 1-octen-3-ol in human embryonic stem cells.
Article in Mycopathologia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 49 citations in OpenAlex.
- Diversity and functions of fungal VOCs with special reference to the multiple bioactivities of the mushroom alcohol.Mycology · 2025Review
- Article
- Effects of Drying Methods and Temperatures on the Quality of Chestnut Flours.Foods (Basel, Switzerland) · 2022Article
- In Vitro Systems for Toxicity Evaluation of Microbial Volatile Organic Compounds on Humans: Current Status and Trends.Journal of fungi (Basel, Switzerland) · 2022Review
- Highly sensitive VOC detectors using insect olfactory receptors reconstituted into lipid bilayers.Science advances · 2021Article
- Volatile Organic Compounds Produced by Human Pathogenic Fungi Are Toxic toFrontiers in fungal biology · 2020Article
- Influence of R and S enantiomers of 1-octen-3-ol on gene expression of Penicillium chrysogenum.Journal of industrial microbiology & biotechnology · 2019Article
- Is Mold Toxicity Really a Problem for Our Patients? Part 2-Nonrespiratory Conditions.Integrative medicine (Encinitas, Calif.) · 2016Article
- Review
- Effects of Three Volatile Oxylipins on Colony Development in Two Species of Fungi and on Drosophila Larval Metamorphosis.Current microbiology · 2015Article
- Review
- A common fungal volatile organic compound induces a nitric oxide mediated inflammatory response in Drosophila melanogaster.Scientific reports · 2014Article
- The effects of fungal volatile organic compounds on bone marrow stromal cells.Canadian journal of microbiology · 2014Article
- Fungal-derived semiochemical 1-octen-3-ol disrupts dopamine packaging and causes neurodegeneration.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Stem cells in drug screening for neurodegenerative disease.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial growth in damp indoor environments has been correlated with risks to human health. This study was aimed to determine the cytotoxicity of 1-octen-3-ol ("mushroom alcohol"), a major fungal volatile organic compound (VOC) associated with mushroom and mold odors. Using an airborne exposure technique, human embryonic stem cells were exposed for 1 h to different concentrations (0-1,000 ppm) of racemic 1-octen-3-ol and its enantiomers, (R)-(-)-1-octen-3-ol and (S)-(+)-1-octen-3-ol. Cytotoxicity was assayed using both the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay and the fluorescently tagged Calcein AM-mediated "live and dead" assay. Racemic 1-octen-3-ol and (S)-(+)-1-octen-3-ol exhibited greater cytotoxicity to the undifferentiated human cell line H1 than did (R)-(-)-1-octen-3-ol. The inhibition concentration 50 (IC(50)) values assessed by the MTS assay for racemic 1-octen-3-ol, (S)-(+)-1-octen-3-ol and (R)-(-)-1-octen-3-ol were, respectively, 109, 98, and 258 ppm. These IC(50) values were 40-80-fold lower than that of vapor phase toluene, an industrial chemical used as a positive control in this study. Our report pioneers the modeling of human embryonic stem cells as an in vitro approach to screen the potential toxicity of fungal VOCs. Human embryonic stem cells exposed to 1-octen-3-ol, and its enantiomers in the vapor phase showed more cytotoxicity than those exposed to toluene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.