ArticlePloS one2011
Newly developed Mg2+-selective fluorescent probe enables visualization of Mg2+ dynamics in mitochondria.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Fluorescence Properties and MgChemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Facile Synthesis of Carbon Dots from Green Resource and Their Application for Selective Determination of MgJournal of fluorescence · 2025Article
- Mrs2-mediated mitochondrial magnesium uptake is essential for the regulation of MCU-mediated mitochondrial CaMitochondrion · 2024Article
- Ratiometric Fluorescent Sensors Illuminate Cellular Magnesium Imbalance in a Model of Acetaminophen-Induced Liver Injury.Journal of the American Chemical Society · 2023Article
- Lanthanide-based luminescent probes for biological magnesium: accessing polyphosphate-bound MgChemical communications (Cambridge, England) · 2023Article
- Transcriptional Control ofInternational journal of molecular sciences · 2022Article
- Magnesium- a Forgotten Element: Phenotypic Variation and Genome Wide Association Study in Turkish Common Bean Germplasm.Frontiers in genetics · 2022Article
- Advances in imaging of understudied ions in signaling: A focus on magnesium.Current opinion in chemical biology · 2020Review
- Magnesium Is a Key Player in Neuronal Maturation and Neuropathology.International journal of molecular sciences · 2019Review
- Highly Selective, Red Emitting BODIPY-Based Fluorescent Indicators for Intracellular MgJournal of materials chemistry. B · 2018Article
- Fluorescent probes for the detection of magnesium ions (MgRSC advances · 2018Review
- A fluorescent activatable probe for imaging intracellular MgOrganic & biomolecular chemistry · 2018Article
- Visualization of long-term MgChemical science · 2017Article
- Coherent and Contradictory Facts, Feats and Fictions Associated with Metal Accumulation in Parkinson's Disease: Epicenter or Outcome, Yet a Demigod Question.Molecular neurobiology · 2017Review
- The Involvement of MgOxidative medicine and cellular longevity · 2017Review
- Mitochondrial Mg(2+) homeostasis decides cellular energy metabolism and vulnerability to stress.Scientific reports · 2016Article
- Visualizing changes in mitochondrial MgChemical science · 2015Article
- Formation of ternary complexes with MgATP: effects on the detection of Mg2+ in biological samples by bidentate fluorescent sensors.Inorganic chemistry · 2014Article
- Bioanalysis of eukaryotic organelles.Chemical reviews · 2013Review
- Inward-rectifying potassium (Kir) channels regulate pacemaker activity in spinal nociceptive circuits during early life.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Mg(2+) plays important roles in numerous cellular functions. Mitochondria take part in intracellular Mg(2+) regulation and the Mg(2+) concentration in mitochondria affects the synthesis of ATP. However, there are few methods to observe Mg(2+) in mitochondria in intact cells. Here, we have developed a novel Mg(2+)-selective fluorescent probe, KMG-301, that is functional in mitochondria. This probe changes its fluorescence properties solely depending on the Mg(2+) concentration in mitochondria under physiologically normal conditions. Simultaneous measurements using this probe together with a probe for cytosolic Mg(2+), KMG-104, enabled us to compare the dynamics of Mg(2+) in the cytosol and in mitochondria. With this method, carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP)-induced Mg(2+) mobilization from mitochondria to the cytosol was visualized. Although a FCCP-induced decrease in the Mg(2+) concentration in mitochondria and an increase in the cytosol were observed both in differentiated PC12 cells and in hippocampal neurons, the time-courses of concentration changes varied with cell type. Moreover, the relationship between mitochondrial Mg(2+) and Parkinson's disease was analyzed in a cellular model of Parkinson's disease by using the 1-methyl-4-phenylpyridinium ion (MPP(+)). A gradual decrease in the Mg(2+) concentration in mitochondria was observed in response to MPP(+) in differentiated PC12 cells. These results indicate that KMG-301 is useful for investigating Mg(2+) dynamics in mitochondria. All animal procedures to obtain neurons from Wistar rats were approved by the ethical committee of Keio University (permit number is 09106-(1)).
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