ArticlePloS one2011
The distribution of phosphatidylinositol 4,5-bisphosphate in acinar cells of rat pancreas revealed with the freeze-fracture replica labeling method.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Subcellular Cartography of the Phosphoinositide Multiverse.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2026Review
- Higher-order transient structures and the principle of dynamic connectivity in membrane signaling.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Nanoscale Phosphoinositide Distribution on Cell Membranes of Mouse Cerebellar Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Long-term depression in neurons involves temporal and ultra-structural dynamics of phosphatidylinositol-4,5-bisphosphate relying on PIP5K, PTEN and PLC.Communications biology · 2023Article
- The distribution of phosphatidylinositol 4,5-bisphosphate in the budding yeast plasma membrane.Histochemistry and cell biology · 2021Article
- Polarized PtdIns(4,5)PProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Regulation of TRPV1 ion channel by phosphoinositide (4,5)-bisphosphate: the role of membrane asymmetry.The Journal of biological chemistry · 2014Article
- Short-chain phosphoinositide partitioning into plasma membrane models.Biophysical journal · 2013Article
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- Polarity and stratification of the epidermis.Seminars in cell & developmental biology · 2012Review
- PDK1 in apical signaling endosomes participates in the rescue of the polarity complex atypical PKC by intermediate filaments in intestinal epithelia.Molecular biology of the cell · 2012Article
- ERα-mediated repression of pro-inflammatory cytokine expression by glucocorticoids reveals a crucial role for TNFα and IL1α in lumen formation and maintenance.Journal of cell science · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidylinositol 4,5-bisphosphate [PI(4,5)P(2)] is a phospholipid that has been implicated in multiple cellular activities. The distribution of PI(4,5)P(2) has been analyzed extensively using live imaging of the GFP-coupled phospholipase C-δ1 pleckstrin homology domain in cultured cell lines. However, technical difficulties have prevented the study of PI(4,5)P(2) in cells of in vivo tissues. We recently developed a method to analyze the nanoscale distribution of PI(4,5)P(2) in cultured cells by using the quick-freezing and freeze-fracture replica labeling method. In principle, this method can be applied to any cell because it does not require the expression of artificial probes. In the present study, we modified the method to study cells of in vivo tissues and applied it to pancreatic exocrine acinar cells of the rat. We found that PI(4,5)P(2) in the plasma membrane is distributed in an equivalent density in the apical and basolateral domains, but exists in a significantly higher concentration in the gap junction. The intracellular organelles did not show labeling for PI(4,5)P(2). The results are novel or different from the reported distribution patterns in cell lines and highlight the importance of studying cells differentiated in vivo.
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