ArticlePloS one2011
Probing the role of protein surface charge in the activation of PrfA, the central regulator of Listeria monocytogenes pathogenesis.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- On the role of cell chaining in the attenuation of aVirulence · 2026Article
- PrfA, the master virulence regulator of Listeria monocytogenes: structural-functional insights, regulatory architecture, and antivirulence strategies.Molecular biology reports · 2026Review
- Large Multicountry Outbreak of Invasive Listeriosis by a Listeria monocytogenes ST394 Clone Linked to Smoked Rainbow Trout, 2020 to 2021.Microbiology spectrum · 2023Article
- Identification of Listeria monocytogenes Genes Contributing to Oxidative Stress Resistance under Conditions Relevant to Host Infection.Infection and immunity · 2021Article
- Prevalence, Pathogenicity, Virulence, Antibiotic Resistance, and Phylogenetic Analysis of Biofilm-ProducingPathogens (Basel, Switzerland) · 2019Article
- Regulation ofMicrobiology spectrum · 2019Review
- Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Attenuating Listeria monocytogenes Virulence by Targeting the Regulatory Protein PrfA.Cell chemical biology · 2016Article
- RNA Helicase Important for Listeria monocytogenes Hemolytic Activity and Virulence Factor Expression.Infection and immunity · 2016Article
- Identification of a peptide-pheromone that enhances Listeria monocytogenes escape from host cell vacuoles.PLoS pathogens · 2015Article
- Strain-Specific Interactions of Listeria monocytogenes with the Autophagy System in Host Cells.PloS one · 2015Article
- Enantioselective regulation of lactate racemization by LarR in Lactobacillus plantarum.Journal of bacteriology · 2015Article
- How Listeria monocytogenes organizes its surface for virulence.Frontiers in cellular and infection microbiology · 2014Review
- Allosteric mutants show that PrfA activation is dispensable for vacuole escape but required for efficient spread and Listeria survival in vivo.Molecular microbiology · 2012Article
- Optimizing the balance between host and environmental survival skills: lessons learned from Listeria monocytogenes.Future microbiology · 2012Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Listeria monocytogenes is a food-borne intracellular bacterial pathogen capable of causing serious human disease. L. monocytogenes survival within mammalian cells depends upon the synthesis of a number of secreted virulence factors whose expression is regulated by the transcriptional activator PrfA. PrfA becomes activated following bacterial entry into host cells where it induces the expression of gene products required for bacterial spread to adjacent cells. Activation of PrfA appears to occur via the binding of a small molecule cofactor whose identity remains unknown. Electrostatic modeling of the predicted PrfA cofactor binding pocket revealed a highly positively charged region with two lysine residues, K64 and K122, located at the edge of the pocket and another (K130) located deep within the interior. Mutational analysis of these residues indicated that K64 and K122 contribute to intracellular activation of PrfA, whereas a K130 substitution abolished protein activity. The requirement of K64 and K122 for intracellular PrfA activation could be bypassed via the introduction of the prfA G145S mutation that constitutively activates PrfA in the absence of cofactor binding. Our data indicate that the positive charge of the PrfA binding pocket contributes to intracellular activation of PrfA, presumably by facilitating binding of an anionic cofactor.
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