ArticlePloS one2011
A novel multiplex real-time PCR for the identification of mycobacteria associated with zoonotic tuberculosis.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- A new diagnostic kit using real-time polymerase chain reaction for the detection of Mycobacterium tuberculosis.AMB Express · 2026Article
- Bovine tuberculosis in Central Ethiopian slaughterhouses and the identification of causative mycobacteria by multiplex real-time PCR.BMC microbiology · 2024Article
- Development of a Multiplex Real-Time PCR Assay for Mycobacterium bovis BCG and Validation in a Clinical Laboratory.Microbiology spectrum · 2021Article
- Quantitative PCR in soil-transmitted helminth epidemiology and control programs: Toward a universal standard.PLoS neglected tropical diseases · 2021Article
- Review
- A rapid and sensitive system for recovery of nucleic acids from Mycobacteria sp. on archived glass slides.BMC microbiology · 2018Article
- Mycobacterium caprae Infection in Captive Borneo Elephant, Japan.Emerging infectious diseases · 2018Article
- Matrixlysis, an improved sample preparation method for recovery of Mycobacteria from animal tissue material.PloS one · 2017Article
- [Identification ofZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2016Article
- Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood.BMC infectious diseases · 2015Article
- Standing of nucleic acid testing strategies in veterinary diagnosis laboratories to uncover Mycobacterium tuberculosis complex members.Frontiers in molecular biosciences · 2014Review
- Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Clinical detection and characterization of bacterial pathogens in the genomics era.Genome medicine · 2014Article
- Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Novel Mycobacterium tuberculosis complex isolate from a wild chimpanzee.Emerging infectious diseases · 2013Article
- Region of difference 4 in alpine Mycobacterium caprae isolates indicates three variants.Journal of clinical microbiology · 2013Article
- SeekTB, a two-stage multiplex real-time-PCR-based method for differentiation of the Mycobacterium tuberculosis complex.Journal of clinical microbiology · 2012Article
- Development and comparison of two assay formats for parallel detection of four biothreat pathogens by using suspension microarrays.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTuberculosis (TB) is the leading cause of death worldwide from a single infectious agent. An ability to detect the Mycobacterium tuberculosis complex (MTC) in clinical material while simultaneously differentiating its members is considered important. This allows for the gathering of epidemiological information pertaining to the prevalence, transmission and geographical distribution of the MTC, including those MTC members associated with zoonotic TB infection in humans. Also differentiating between members of the MTC provides the clinician with inherent MTC specific drug susceptibility profiles to guide appropriate chemotherapy. METHODOLOGY/PRINCIPAL
findingsThe aim of this study was to develop a multiplex real-time PCR assay using novel molecular targets to identify and differentiate between the phylogenetically closely related M. bovis, M. bovis BCG and M. caprae. The lpqT gene was explored for the collective identification of M. bovis, M. bovis BCG and M. caprae, the lepA gene was targeted for the specific identification of M. caprae and a Region of Difference 1 (RD1) assay was incorporated in the test to differentiate M. bovis BCG. The multiplex real-time PCR assay was evaluated on 133 bacterial strains and was determined to be 100% specific for the members of the MTC targeted. CONCLUSIONS/SIGNIFICANCE: The multiplex real-time PCR assay developed in this study is the first assay described for the identification and simultaneous differentiation of M. bovis, M. bovis BCG and M. caprae in one internally controlled reaction. Future validation of this multiplex assay should demonstrate its potential in the rapid and accurate diagnosis of TB caused by these three mycobacteria. Furthermore, the developed assay may be used in conjunction with a recently described multiplex real-time PCR assay for identification of the MTC and simultaneous differentiation of M. tuberculosis, M. canettii resulting in an ability to differentiate five of the eight members of the MTC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.