ArticlePloS one2011
Degenerate wave and capacitive coupling increase human MSC invasion and proliferation while reducing cytotoxicity in an in vitro wound healing model.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 79 citations in OpenAlex.
- Evaluation of Pulsed Electromagnetic Field Effects: A Systematic Review and Meta-Analysis on Highlights of Two Decades of Research In Vitro Studies.BioMed research international · 2021Pooled it
- Review
- Enhancing cell motility via non-contact capacitively coupled electrostatic field.Scientific reports · 2024Article
- Stem Cells in Bone Tissue Engineering: Progress, Promises and Challenges.Stem cell reviews and reports · 2024Review
- Role of biophysical stimulation in multimodal management of vertebral compression fractures.Computational and structural biotechnology journal · 2023Review
- How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study.Scientific reports · 2022Review
- Interfaces Based on Laser-Structured Arrays of Carbon Nanotubes with Albumin for Electrical Stimulation of Heart Cell Growth.Polymers · 2022Article
- Exposure of primary osteoblasts to combined magnetic and electric fields induced spatiotemporal endochondral ossification characteristic gene- and protein expression profiles.Journal of experimental orthopaedics · 2022Article
- Long-term stimulation with alternating electric fields modulates the differentiation and mineralization of human pre-osteoblasts.Frontiers in physiology · 2022Article
- Effect of mesenchymal stem cells versus aloe vera on healing of deep second-degree burn.Stem cell investigation · 2021Article
- Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus.Stem cell research & therapy · 2020Article
- Electrical stimulation in bone tissue engineering treatments.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2020Review
- Liquid Metal Enabled Electrobiology: A New Frontier to Tackle Disease Challenges.Micromachines · 2018Review
- Combined treatment with electrical stimulation and insulin-like growth factor-1 promotes bone regeneration in vitro.PloS one · 2018Article
- The effect of capacitively coupled (CC) electrical stimulation on human disc nucleus pulposus cells and the relationship between CC and BMP-7.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2017Article
- New cosurface capacitive stimulators for the development of active osseointegrative implantable devices.Scientific reports · 2016Article
- Modulation of Osteogenesis in MC3T3-E1 Cells by Different Frequency Electrical Stimulation.PloS one · 2016Article
- Effects of electrical stimulation on rat limb regeneration, a new look at an old model.Scientific reports · 2015Article
- Current progress in use of adipose derived stem cells in peripheral nerve regeneration.World journal of stem cells · 2015Review
- Role of mesenchymal stem cells in bone regeneration and fracture repair: a review.International orthopaedics · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Non-unions pose complications in fracture management that can be treated using electrical stimulation (ES). Bone marrow mesenchymal stem cells (BMMSCs) are essential in fracture healing; however, the effect of different clinical ES waveforms on BMMSCs cellular activities remains unknown. We compared the effects of direct current (DC), capacitive coupling (CC), pulsed electromagnetic field (PEMF) and degenerate wave (DW) on cellular activities including cytotoxicity, proliferation, cell-kinetics and apoptosis by stimulating human-BMMSCs 3 hours a day, up to 5 days. In addition, migration and invasion were assessed using fluorescence microscopy and by quantifying gene and protein expression. We found that DW had the greatest proliferative and least apoptotic and cytotoxic effects compared to other waveforms. DC, DW and CC stimulations resulted in a higher number of cells in S phase and G(2)/M phase as shown by cell cycle analysis. CC and DW caused more cells to invade collagen and showed increased MMP-2 and MT1-MMP expression. DC increased cellular migration in a scratch-wound assay and all ES waveforms enhanced expression of migratory genes with DC having the greatest effect. All ES treated cells showed similar progenitor potential as determined by MSC differentiation assay. All above findings were shown to be statistically significant (p<0.05). We conclude that ES can influence BMMSCs activities, especially DW and CC, which show greater invasion and higher cell proliferation compared to other types of ES. Application of DW or CC to the fracture site may help in the recruitment of BMMSCs to the wound that may enhance rate of bone healing at the fracture site.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.