ArticleNature structural & molecular biology2011
The structural basis of RNA-catalyzed RNA polymerization.
Article in Nature structural & molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 0 citations in OpenAlex.
- A universal Fab targeting a conserved U1A-RNA epitope for RNA structure determination by cryo-EM.Nucleic acids research · 2026Article
- Multiomic characterization of RNA microenvironments by oligonucleotide-mediated proximity-interactome mapping.Nature methods · 2024Article
- Architecture of an RNA polymerase ribozyme illuminates the RNA World.Trends in genetics : TIG · 2024Article
- Cryo-EM structure and functional landscape of an RNA polymerase ribozyme.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A ribozyme that uses lanthanides as cofactor.Nucleic acids research · 2023Article
- Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit.Biology · 2023Article
- Article
- Kinetic Effects of β,γ-Modified Deoxynucleoside 5'-Triphosphate Analogues on RNA-Catalyzed Polymerization of DNA.Biochemistry · 2021Article
- Dynamics of the vesicles composed of fatty acids and other amphiphile mixtures: unveiling the role of fatty acids as a model protocell membrane.Biophysical reviews · 2020Article
- In vitro selection of ribozyme ligases that use prebiotically plausible 2-aminoimidazole-activated substrates.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- RNA Regulatory Networks as a Control of Stochasticity in Biological Systems.Frontiers in genetics · 2019Review
- The NTP binding site of the polymerase ribozyme.Nucleic acids research · 2018Article
- Possible Emergence of Sequence Specific RNA Aminoacylation via Peptide Intermediary to Initiate Darwinian Evolution and Code Through Origin of Life.Life (Basel, Switzerland) · 2018Article
- Models of Replicator Proliferation Involving Differential Replicator Subunit Stability.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2018Article
- Crystal structure of a DNA aptamer bound to PvLDH elucidates novel single-stranded DNA structural elements for folding and recognition.Scientific reports · 2016Article
- Crystal structure of a DNA catalyst.Nature · 2016Article
- Reviving the RNA World: An Insight into the Appearance of RNA Methyltransferases.Frontiers in genetics · 2016Review
- Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display.Nature methods · 2015Article
- The ribosome challenge to the RNA world.Journal of molecular evolution · 2015Review
- What RNA World? Why a Peptide/RNA Partnership Merits Renewed Experimental Attention.Life (Basel, Switzerland) · 2015Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Early life presumably required polymerase ribozymes capable of replicating RNA. Known polymerase ribozymes best approximating such replicases use as their catalytic engine an RNA-ligase ribozyme originally selected from random RNA sequences. Here we report 3.15-Å crystal structures of this ligase trapped in catalytically viable preligation states, with the 3'-hydroxyl nucleophile positioned for in-line attack on the 5'-triphosphate. Guided by metal- and solvent-mediated interactions, the 5'-triphosphate hooks into the major groove of the adjoining RNA duplex in an unanticipated conformation. Two phosphates and the nucleophile jointly coordinate an active-site metal ion. Atomic mutagenesis experiments demonstrate that active-site nucleobase and hydroxyl groups also participate directly in catalysis, collectively playing a role that in proteinaceous polymerases is performed by a second metal ion. Thus artificial ribozymes can use complex catalytic strategies that differ markedly from those of analogous biological enzymes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.