ArticleFly
An efficient method for recombineering GAL4 and QF drivers.
Article in Fly. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Octopamine receptors inFrontiers in molecular neuroscience · 2026Article
- Serial Recombineering Cloning to Build Selectable and Tagged Genomic P[acman] BAC Clones for Selection Transgenesis and Functional Gene Analysis using Drosophila melanogaster.Current protocols · 2023Article
- Developmental loss of neurofibromin across distributed neuronal circuits drives excessive grooming in Drosophila.PLoS genetics · 2020Article
- The Q-System: A Versatile Expression System for Drosophila.Methods in molecular biology (Clifton, N.J.) · 2016Review
- Chemical mutagens, transposons, and transgenes to interrogate gene function in Drosophila melanogaster.Methods (San Diego, Calif.) · 2014Review
- The genetic analysis of functional connectomics in Drosophila.Advances in genetics · 2012Review
- Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neural circuit mapping and manipulation are facilitated by independent control of gene expression in pre- and post-synaptic neurons. The GAL4/UAS and Q binary transcription systems have the potential to provide this capability. Of particular use in neural circuit mapping would be GAL4 and QF drivers specific for neurotransmitters and neurotransmitter receptors. Recently available Drosophila genomic BAC libraries make recombineering large genes including those specific for neurotransmitters and neurotransmitter receptors feasible. Here the functionality of cassettes that allow efficient recombineering of GAL4 and QF drivers based on kanamycin selection is demonstrated in Drosophila. The cassettes should, however, be generalizable for recombineering in other species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.