ArticleJournal of bacteriology2011
Nonclassical protein secretion by Bacillus subtilis in the stationary phase is not due to cell lysis.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 108 citations in OpenAlex.
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- Secretome Analysis of the Plant Biostimulant Bacteria StrainsInternational journal of molecular sciences · 2022Article
- ASPIRER: a new computational approach for identifying non-classical secreted proteins based on deep learning.Briefings in bioinformatics · 2022Article
- Extracellular Glycolytic Activities in Root EndophyticMicroorganisms · 2022Article
- An overview and future prospects of recombinant protein production in Bacillus subtilis.Applied microbiology and biotechnology · 2021Review
- Global mapping of Salmonella enterica-host protein-protein interactions during infection.Cell host & microbe · 2021Article
- Proteomic study of Desulfovibrio ferrophilus IS5 reveals overexpressed extracellular multi-heme cytochrome associated with severe microbiologically influenced corrosion.Scientific reports · 2021Article
- Moonlighting inMicroorganisms · 2021Review
- "Applied and environmental microbiology · 2021Article
- The Phosphoarginine Phosphatase PtpB fromCells · 2021Article
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- Streptococcus pneumoniae aminopeptidase N contributes to bacterial virulence and elicits a strong innate immune response through MAPK and PI3K/AKT signaling.Journal of microbiology (Seoul, Korea) · 2020Article
- The Russian Doll Model: How Bacteria Shape Successful and Sustainable Inter-Kingdom Relationships.Frontiers in microbiology · 2020Review
- The Ser/Thr protein kinase PrkC imprints phenotypic memory inThe Journal of biological chemistry · 2019Article
- A new approach for analyzing an adhesive bacterial protein in the mouse gastrointestinal tract using optical tissue clearing.Scientific reports · 2019Article
- Review
- SecA inhibitors as potential antimicrobial agents: differential actions on SecA-only and SecA-SecYEG protein-conducting channels.FEMS microbiology letters · 2018Review
- Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme.Microbial cell factories · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The carboxylesterase Est55 has been cloned and expressed in Bacillus subtilis strains. Est55, which lacks a classical, cleavable N-terminal signal sequence, was found to be secreted during the stationary phase of growth such that there is more Est55 in the medium than inside the cells. Several cytoplasmic proteins were also secreted in large amounts during late stationary phase, indicating that secretion in B. subtilis is not unique to Est55. These proteins, which all have defined cytoplasmic functions, include GroEL, DnaK, enolase, pyruvate dehydrogenase subunits PdhB and PdhD, and SodA. The release of Est55 and those proteins into the growth medium is not due to gross cell lysis, a conclusion that is supported by several lines of evidence: constant cell density and secretion in the presence of chloramphenicol, constant viability count, the absence of EF-Tu and SecA in the culture medium, and the lack of effect of autolysin-deficient mutants. The shedding of these proteins by membrane vesicles into the medium is minimal. More importantly, we have identified a hydrophobic α-helical domain within enolase that contributes to its secretion. Thus, upon the genetic deletion or replacement of a potential membrane-embedding domain, the secretion of plasmid gene-encoded mutant enolase is totally blocked, while the wild-type chromosomal enolase is secreted normally in the same cultures during the stationary phase, indicating differential specificity. We conclude that the secretion of Est55 and several cytoplasmic proteins without signal peptides in B. subtilis is a general phenomenon and is not a consequence of cell lysis or membrane shedding; instead, their secretion is through a process(es) in which protein domain structure plays a contributing factor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.