ArticleApplied and environmental microbiology2011
Mechanisms of fluoroquinolone resistance in Escherichia coli isolates from food-producing animals.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 79 citations in OpenAlex.
- Molecular Screening and Antibiogram Profile of Multidrug-Resistant Enteropathogenic Escherichia coli Isolated From Retail Chicken Meat.Veterinary medicine and science · 2026Article
- Consequences of benzalkonium chloride tolerance for selection dynamics and de novo resistance evolution driven by antibiotics.npj antimicrobials and resistance · 2026Article
- Role of the Bacterial Amyloid-like Hfq in Fluoroquinolone Fluxes.Microorganisms · 2023Article
- Genetic characterization of ESBL-producing and ciprofloxacin-resistantFrontiers in microbiology · 2023Article
- A pharmacodynamic investigation to assess the synergism of orbifloxacin and propyl gallate againstFrontiers in pharmacology · 2022Article
- Biological Effects of Quinolones: A Family of Broad-Spectrum Antimicrobial Agents.Molecules (Basel, Switzerland) · 2021Review
- Antimicrobial Resistance Genes in ESBL-ProducingAntibiotics (Basel, Switzerland) · 2021Article
- Prevalence of Multidrug-Resistant Foodborne Pathogens and Indicator Bacteria from Edible Offal and Muscle Meats in Nashville, Tennessee.Foods (Basel, Switzerland) · 2020Article
- Machine learning with random subspace ensembles identifies antimicrobial resistance determinants from pan-genomes of three pathogens.PLoS computational biology · 2020Article
- Synergistic Activity of Fluoroquinolones Combining with Artesunate Against Multidrug-ResistantMicrobial drug resistance (Larchmont, N.Y.) · 2020Article
- Effects of a previously selected antibiotic resistance on mutations acquired during development of a second resistance in Escherichia coli.BMC genomics · 2019Article
- Whole-Genome Sequencing and Genetic Analysis Reveal Novel Stress Responses to Individual Constituents of Essential Oils in Escherichia coli.Applied and environmental microbiology · 2018Article
- Extra-Intestinal Fluoroquinolone-Resistant Escherichia coli Strains Isolated from Meat.BioMed research international · 2018Article
- ISInfection and drug resistance · 2018Article
- Mechanisms of quinolone resistance inJournal of veterinary science · 2017Article
- The Global Regulatory Cyclic AMP Receptor Protein (CRP) Controls Multifactorial Fluoroquinolone Susceptibility in Salmonella enterica Serovar Typhimurium.Antimicrobial agents and chemotherapy · 2017Article
- Quinolone co-resistance in ESBL- or AmpC-producing Escherichia coli from an Indian urban aquatic environment and their public health implications.Environmental science and pollution research international · 2016Article
- The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.Clinical microbiology reviews · 2015Review
- The novel immunotherapeutic oligodeoxynucleotide IMT504 protects neutropenic animals from fatal Pseudomonas aeruginosa bacteremia and sepsis.Antimicrobial agents and chemotherapy · 2015Article
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eleven multidrug-resistant Escherichia coli isolates (comprising 6 porcine and 5 bovine field isolates) displaying fluoroquinolone (FQ) resistance were selected from a collection obtained from the University Veterinary Hospital (Dublin, Ireland). MICs of nalidixic acid and ciprofloxacin were determined by Etest. All showed MICs of nalidixic acid of >256 μg/ml and MICs of ciprofloxacin ranging from 4 to >32 μg/ml. DNA sequencing was used to identify mutations within the quinolone resistance-determining regions of target genes, and quantitative real-time PCR (qRT-PCR) was used to evaluate the expression of the major porin, OmpF, and component genes of the AcrAB-TolC efflux pump and its associated regulatory loci. Decreased MIC values to nalidixic acid and/or ciprofloxacin were observed in the presence of the efflux pump inhibitor phenylalanine-arginine-β-naphthylamide (PAβN) in some but not all isolates. Several mutations were identified in genes coding for quinolone target enzymes (3 to 5 mutations per strain). All isolates harbored GyrA amino acid substitutions at positions 83 and 87. Novel GyrA (Asp87 → Ala), ParC (Ser80 → Trp), and ParE (Glu460 → Val) substitutions were observed. The efflux activity of these isolates was evaluated using a semiautomated ethidium bromide (EB) uptake assay. Compared to wild-type E. coli K-12 AG100, isolates accumulated less EB, and in the presence of PAβN the accumulation of EB increased. Upregulation of the acrB gene, encoding the pump component of the AcrAB-TolC efflux pump, was observed in 5 of 11 isolates, while 10 isolates showed decreased expression of OmpF. This study identified multiple mechanisms that likely contribute to resistance to quinolone-based drugs in the field isolates studied.
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