ArticleThe Plant cell2011
Combined noninvasive imaging and modeling approaches reveal metabolic compartmentation in the barley endosperm.
Article in The Plant cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 76 citations in OpenAlex.
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- A holistic visualization for quality of Chinese materia medica: Structural and metabolic visualization by magnetic resonance imaging.Journal of pharmaceutical analysis · 2024Review
- The diversity of substrates for plant respiration and how to optimize their use.Plant physiology · 2023Review
- Grafting enhances drought tolerance by regulating and mobilizing proteome, transcriptome and molecular physiology in okra genotypes.Frontiers in plant science · 2023Article
- Enhanced nucleotide analysis enables the quantification of deoxynucleotides in plants and algae revealing connections between nucleoside and deoxynucleoside metabolism.The Plant cell · 2021Article
- Analysis of Nucleosides and Nucleotides in Plants: An Update on Sample Preparation and LC-MS Techniques.Cells · 2021Review
- Plant Single-Cell Metabolomics-Challenges and Perspectives.International journal of molecular sciences · 2020Review
- FLOURY ENDOSPERM15 encodes a glyoxalase I involved in compound granule formation and starch synthesis in rice endosperm.Plant cell reports · 2019Article
- Assessment of Pollen Viability for Wheat.Frontiers in plant science · 2019Article
- Micro Imaging Displays the Sucrose Landscape within and along Its Allocation Pathways.Plant physiology · 2018Article
- Seed tissue and nutrient partitioning, a case for the nucellus.Plant reproduction · 2018Review
- Proteomic analysis of heat stress resistance of cucumber leaves when grafted onto Momordica rootstock.Horticulture research · 2018Article
- An Integrated "Multi-Omics" Comparison of Embryo and Endosperm Tissue-Specific Features and Their Impact on Rice Seed Quality.Frontiers in plant science · 2017Article
- Plastidic phosphoglucomutase and ADP-glucose pyrophosphorylase mutants impair starch synthesis in rice pollen grains and cause male sterility.Journal of experimental botany · 2016Article
- Plant Microbe Interactions in Post Genomic Era: Perspectives and Applications.Frontiers in microbiology · 2016Review
- Metabolic Architecture of the Cereal Grain and Its Relevance to Maximize Carbon Use Efficiency.Plant physiology · 2015Article
- Spatially Resolved Plant Metabolomics: Some Potentials and Limitations of Laser-Ablation Electrospray Ionization Mass Spectrometry Metabolite Imaging.Plant physiology · 2015Article
- Metabolite fingerprinting of pennycress (Thlaspi arvense L.) embryos to assess active pathways during oil synthesis.Journal of experimental botany · 2015Article
- Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape during in Vitro Culture.Plant physiology · 2015Article
- Influence of oxygen deficiency and the role of specific amino acids in cryopreservation of garlic shoot tips.BMC biotechnology · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The starchy endosperm of cereals is a priori taken as a metabolically uniform tissue. By applying a noninvasive assay based on (13)C/(1)H-magnetic resonance imaging (MRI) to barley (Hordeum vulgare) grains, we uncovered metabolic compartmentation in the endosperm. (13)C-Suc feeding during grain filling showed that the primary site of Ala synthesis was the central region of the endosperm, the part of the caryopsis experiencing the highest level of hypoxia. Region-specific metabolism in the endosperm was characterized by flux balance analysis (FBA) and metabolite profiling. FBA predicts that in the central region of the endosperm, the tricarboxylic acid cycle shifts to a noncyclic mode, accompanied by elevated glycolytic flux and the accumulation of Ala. The metabolic compartmentation within the endosperm is advantageous for the grain's carbon and energy economy, with a prominent role being played by Ala aminotransferase. An investigation of caryopses with a genetically perturbed tissue pattern demonstrated that Ala accumulation is a consequence of oxygen status, rather than being either tissue specific or dependent on the supply of Suc. Hence the (13)C-Ala gradient can be used as an in vivo marker for hypoxia. The combination of MRI and metabolic modeling offers opportunities for the noninvasive analysis of metabolic compartmentation in plants.
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