ArticleMolecular and biochemical parasitology2011
The primase domain of PfPrex is a proteolytically matured, essential enzyme of the apicoplast.
Article in Molecular and biochemical parasitology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Acyl Carrier Protein is Essential for Apicoplast Biogenesis in Malaria Parasites Independent of Fatty Acid Synthesis.bioRxiv : the preprint server for biology · 2026Article
- Malaria parasites require a divergent heme oxygenase for apicoplast gene expression and biogenesis.eLife · 2024Article
- Malaria parasites require a divergent heme oxygenase for apicoplast gene expression and biogenesis.bioRxiv : the preprint server for biology · 2024Article
- A Plasmodium apicoplast-targeted unique exonuclease/FEN exhibits interspecies functional differences attributable to an insertion that alters DNA-binding.Nucleic acids research · 2024Article
- Apicoplast-Resident Processes: Exploiting the Chink in the Armour ofAdvances in pharmacological and pharmaceutical sciences · 2024Review
- Reclassification of family A DNA polymerases reveals novel functional subfamilies and distinctive structural features.Nucleic acids research · 2023Article
- Transient State Kinetics ofBiochemistry · 2022Article
- Replication and partitioning of the apicoplast genome of Toxoplasma gondii is linked to the cell cycle and requires DNA polymerase and gyrase.International journal for parasitology · 2021Article
- The proofreading activity of Pfprex from Plasmodium falciparum can prevent mutagenesis of the apicoplast genome by oxidized nucleotides.Scientific reports · 2020Article
- The Dictyostelium discoideum homologue of Twinkle, Twm1, is a mitochondrial DNA helicase, an active primase and promotes mitochondrial DNA replication.BMC molecular biology · 2018Article
- SSP3 is a novel Plasmodium yoelii sporozoite surface protein with a role in gliding motility.Infection and immunity · 2014Article
- Enzymes involved in plastid-targeted phosphatidic acid synthesis are essential for Plasmodium yoelii liver-stage development.Molecular microbiology · 2014Article
- Perturbations of Plasmodium Puf2 expression and RNA-seq of Puf2-deficient sporozoites reveal a critical role in maintaining RNA homeostasis and parasite transmissibility.Cellular microbiology · 2013Article
- Lateral gene transfer of family A DNA polymerases between thermophilic viruses, aquificae, and apicomplexa.Molecular biology and evolution · 2013Article
- The HU protein is important for apicoplast genome maintenance and inheritance in Toxoplasma gondii.Eukaryotic cell · 2012Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The apicoplast of Plasmodium is an essential organelle with its own circular genome that must be faithfully replicated and segregated to its progeny during parasite sporogony and schizogony. DNA replication proteins are not encoded by its genome. Instead, the replication machinery must be imported from nuclear-encoded genes. A likely apicoplast DNA replication factor, PfPrex, bears a bipartite leader sequence for apicoplast trafficking and contains several DNA replication-related enzymatic domains. Here we analyze the domain structure of PfPrex and examine its trafficking and maturation within the parasite. A minimal primase domain of PfPrex is shown to contain functional zinc-binding and TOPRIM-fold domains, which in a recombinant form are sufficient to produce RNA primers from a single-stranded DNA template. PfPrex is shown to be extensively proteolytically matured within the parasite, which effectively separates its functional domains. Gene targeting attempts to knockout the Plasmodium yoelii ortholog of Prex were unsuccessful, indicating the apparent essentiality of this protein to the parasite. Finally, overexpression in Plasmodium falciparum of PfPrex's trafficking and primase sequences yielded specific and dynamic localization to foci within the apicoplast. Taken together, these observations strongly suggest an essential role of PfPrex primase in the production of RNA primers for lagging strand DNA synthesis of the apicoplast genome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.