ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2011
Non-invasive sample collection for respiratory virus testing by multiplex PCR.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- BCSH/BSBMT/UK clinical virology network guideline: diagnosis and management of common respiratory viral infections in patients undergoing treatment for haematological malignancies or stem cell transplantation.British journal of haematology · 2016Guideline
- Nasal microbiome and phageome profiles are associated with prospective respiratory viral infection risk in school-age children.The Journal of allergy and clinical immunology · 2026Article
- A decade of clinical microbiology: top 10 advances in 10 years: what every infection preventionist and antimicrobial steward should know.Antimicrobial stewardship & healthcare epidemiology : ASHE · 2024Review
- Characterisation of RSV infections in children without chronic diseases aged 0-36 months during the post-COVID-19 winter season 2022/2023.Frontiers in pediatrics · 2024Article
- Using Discarded Facial Tissues to Monitor and Diagnose Viral Respiratory Infections.Emerging infectious diseases · 2023Article
- The surge of RSV and other respiratory viruses among children during the second COVID-19 pandemic winter season.Frontiers in pediatrics · 2023Article
- Advances in the Rapid Diagnostic of Viral Respiratory Tract Infections.Frontiers in cellular and infection microbiology · 2022Review
- SARS-CoV-2 in saliva, viremia and seroprevalence for COVID-19 surveillance at a single hematopoietic stem cell transplantation center: a prospective cohort study.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Article
- Respiratory Infections in Children During a Covid-19 Pandemic Winter.Frontiers in pediatrics · 2021Article
- Validation of SARS-CoV-2 detection across multiple specimen types.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020Article
- A Practical Approach to the Management of Cancer Patients During the Novel Coronavirus Disease 2019 (COVID-19) Pandemic: An International Collaborative Group.The oncologist · 2020Review
- Article
- Respiratory virus detection and co-infection in children and adults in a large Australian hospital in 2009-2015.Journal of paediatrics and child health · 2018Observational
- Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction.Molecular medicine reports · 2018Article
- Asthma exacerbations in a subtropical area and the role of respiratory viruses: a cross-sectional study.BMC pulmonary medicine · 2018Article
- Bayesian evidence and epidemiological implications of environmental contamination from acute respiratory infection in long-term care facilities.Epidemiology and infection · 2018Article
- The association between anterior nares and nasopharyngeal microbiota in infants hospitalized for bronchiolitis.Microbiome · 2018Article
- Aerosol Sampling in a Hospital Emergency Room Setting: A Complementary Surveillance Method for the Detection of Respiratory Viruses.Frontiers in public health · 2018Article
- Diagnosing Viral and Atypical Pathogens in the Setting of Community-Acquired Pneumonia.Clinics in chest medicine · 2017Review
- Community Surveillance of Respiratory Viruses Among Families in the Utah Better Identification of Germs-Longitudinal Viral Epidemiology (BIG-LoVE) Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2015Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundIdentifying respiratory pathogens within populations is difficult because invasive sample collection, such as with nasopharyngeal aspirate (NPA), is generally required. PCR technology could allow for non-invasive sampling methods.
objectiveEvaluate the utility of non-invasive sample collection using anterior nare swabs and facial tissues for respiratory virus detection by multiplex PCR. STUDY
designChildren aged 1 month-17 years evaluated in a pediatric emergency department for respiratory symptoms had a swab, facial tissue, and NPA sample collected. All samples were tested for respiratory viruses by multiplex PCR. Viral detection rates were calculated for each collection method. Sensitivity and specificity of swabs and facial tissues were calculated using NPA as the gold standard.
results285 samples from 95 children were evaluated (92 swab-NPA pairs, 91 facial tissue-NPA pairs). 91% of NPA, 82% of swab, and 77% of tissue samples were positive for ≥1 virus. Respiratory syncytial virus (RSV) and human rhinovirus (HRV) were most common. Overall, swabs were positive for 74% of virus infections, and facial tissues were positive for 58%. Sensitivity ranged from 17 to 94% for swabs and 33 to 84% for tissues. Sensitivity was highest for RSV (94% swabs and 84% tissues). Specificity was ≥95% for all viruses except HRV for both collection methods.
conclusionsSensitivity of anterior nare swabs and facial tissues in the detection of respiratory viruses by multiplex PCR varied by virus type. Given its simplicity and specificity, non-invasive sampling for PCR testing may be useful for conducting epidemiologic or surveillance studies in settings where invasive testing is impractical or not feasible.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.