ArticleBMC microbiology2011
In Mycoplasma hominis the OppA-mediated cytoadhesion depends on its ATPase activity.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 29 citations in OpenAlex.
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- Analysis of the Adhesive-Invasive Potential of Two Morphologically Different Types of Mycoplasma hominis Colonies.Bulletin of experimental biology and medicine · 2022Article
- Thymidine utilisation pathway is a novel phenotypic switch ofJournal of medical microbiology · 2022Article
- Virulence factors ofFrontiers in microbiology · 2022Article
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- Review
- Deletion of the oligopeptide transporter Lmo2193 decreases the virulence of Listeria monocytogenes.Journal of veterinary science · 2020Article
- Mbov_0503 Encodes a Novel Cytoadhesin that FacilitatesMicroorganisms · 2020Article
- Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING).BMC genomics · 2018Article
- Validation of a novel Mho microarray for a comprehensive characterisation of the Mycoplasma hominis action in HeLa cell infection.PloS one · 2017Article
- Phyllosphere Metaproteomes of Trees from the Brazilian Atlantic Forest Show High Levels of Functional Redundancy.Microbial ecology · 2017Article
- Genetic loci of Mycoplasma agalactiae involved in systemic spreading during experimental intramammary infection of sheep.Veterinary research · 2016Article
- Differential expression of Spiroplasma citri surface protein genes in the plant and insect hosts.BMC microbiology · 2016Article
- Changes in pathogenicity and immunogenicity of Mycoplasma mycoides subsp. mycoides strains revealed by comparative genomics analysis.Scientific reports · 2016Article
- Genome Sequences of Seven Mycoplasma hyosynoviae Strains Isolated from the Joint Tissue of Infected Swine (Sus scrofa).Genome announcements · 2014Article
- Surface glycosaminoglycans mediate adherence between HeLa cells and Lactobacillus salivarius Lv72.BMC microbiology · 2013Article
- Host cell responses to persistent mycoplasmas--different stages in infection of HeLa cells with Mycoplasma hominis.PloS one · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn Mycoplasma hominis, a facultative human pathogen of the human genital tract, OppA, the substrate-binding domain of the oligopeptide permease, is a multifunctional protein involved in nutrition uptake, cytoadhesion and hydrolysis of extracellular ATP.
resultsTo map the function-related protein regions the ATPase activity and adhesive behavior of OppA mutants were analyzed. Mutations of the Walker BA motifs resulted in an inhibition of up to 8% of the OppA ATPase activity, whereas deletion of the N-terminal CS1 or the CS2 region, structural motifs that are conserved in bacterial OppA proteins, reduced ATPase activity to 60% and deletion of CS3, the third conserved region adjacent to the Walker B motif led to a reduction to 42% ATPase activity. Interestingly, adhesion of the OppA mutants to immobilized HeLa cells demonstrated that two distal regions are mainly involved in adherence of OppA: the CS1 region, deletion of which led to 35% of the cytoadhesion, and the Walker BA with the adjacent upstream region CS3, deletion of which led to 25% of the cytoadhesion. The influence of the ATPase activity on the adherence of M. hominis to HeLa cells was confirmed by the use of ATPase inhibitors which reduced mycoplasmal cytoadhesion to 50%.
conclusionsThese findings suggest that the OppA-mediated cytoadherence of Mycoplasma hominis depends on both, the topology of the neighbouring CS1 and ATPase domain regions and the functionality of the ecto-ATPase activity in addition.
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