ArticleAnalytical chemistry2011
Synthesis and characterization of SIRT6 protein coated magnetic beads: identification of a novel inhibitor of SIRT6 deacetylase from medicinal plant extracts.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 85 citations in OpenAlex.
- Rapid screening of α-amylase inhibitors from Aloe vera based on polydopamine/L-cysteine bifunctionalized magnetic mesoporous silica immobilized α-amylase.Analytical and bioanalytical chemistry · 2025Article
- Histone Deacetylase Inhibitors Promote the Anticancer Activity of Cisplatin: Mechanisms and Potential.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Affinity Ultrafiltration Mass Spectrometry for Screening Active Ingredients in Traditional Chinese Medicine: A Review of the Past Decade (2014-2024).Molecules (Basel, Switzerland) · 2025Review
- SIRT6 in Regulation of Mitochondrial Damage and Associated Cardiac Dysfunctions: A Possible Therapeutic Target for CVDs.Cardiovascular toxicology · 2024Review
- Sirtuins as Players in the Signal Transduction ofInternational journal of molecular sciences · 2024Review
- The Role of Increased Expression of Sirtuin 6 in the Prevention of Premature Aging Pathomechanisms.International journal of molecular sciences · 2023Review
- Screening of Monoamine Oxidase Inhibitors from Seeds ofPlants (Basel, Switzerland) · 2023Article
- A preparation strategy for protein-oriented immobilized silica magnetic beads with Spy chemistry for ligand fishing.Journal of pharmaceutical analysis · 2022Article
- Fast screening of tyrosinase inhibitors from traditional Chinese medicinal plants by ligand fishing in combination with in situ fluorescent assay.Analytical and bioanalytical chemistry · 2022Article
- Article
- Naturally occurring small molecule compounds that target histone deacetylases and their potential applications in cancer therapy.The Journal of antibiotics · 2021Review
- Screening of SIRT6 inhibitors and activators: A novel activator has an impact on breast cancer cells.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2021Article
- The synthesis and characterization of Bri2 BRICHOS coated magnetic particles and their application to protein fishing: Identification of novel binding proteins.Journal of pharmaceutical and biomedical analysis · 2021Article
- Emerging roles of SIRT6 in human diseases and its modulators.Medicinal research reviews · 2021Review
- Recent advances in screening active components from natural products based on bioaffinity techniques.Acta pharmaceutica Sinica. B · 2020Review
- Biological and catalytic functions of sirtuin 6 as targets for small-molecule modulators.The Journal of biological chemistry · 2020Review
- Natural Products as Modulators of Sirtuins.Molecules (Basel, Switzerland) · 2020Review
- Magnetic beads-based neuraminidase enzyme microreactor as a drug discovery tool for screening inhibitors from compound libraries and fishing ligands from natural products.Journal of chromatography. A · 2018Article
- Natural polyphenols as sirtuin 6 modulators.Scientific reports · 2018Article
- Ligand Fishing: A Remarkable Strategy for Discovering Bioactive Compounds from Complex Mixture of Natural Products.Molecules (Basel, Switzerland) · 2016Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
SIRT6 is a histone deacetylase that has been proposed as a potential therapeutic target for metabolic disorders and the prevention of age-associated diseases. Thus, the identification of compounds that modulate SIRT6 activity could be of great therapeutic importance. The aim of this study was to develop a screening method for the identification of novel modulators of SIRT6 from a natural plant extract. We immobilized SIRT6 onto the surface of magnetic beads, and assessed SIRT6 enzymatic activity on synthetic acetylated histone tails (H3K9Ac) by measuring products of the deacetylation process. The SIRT6 coated magnetic beads were then suspended in fenugreek seed extract (Trigonella foenum-graecum) as a bait to identify active ligands that suppress SIRT6 activity. While the entire extract also inhibited SIRT6 activity in a cell-based assay, the inhibitory effect of two flavonoids from this extract, quercetin and vitexin, was only detected in vitro. This is the first report on the use of protein-coated magnetic beads for the identification of an active ligand from a botanical matrix, and it sets the basis for the de novo identification of SIRT6 modulators from complex biological mixtures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.