ArticlePlant cell reports2011
Improved Agrobacterium-mediated transformation of cowpea via sonication and vacuum infiltration.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed.
- Development of a High-Efficiency Hairy Root Transformation System for Diverse Cowpea (Plants (Basel, Switzerland) · 2026Article
- Geminiviral-CR-gRNA expressed in cowpea efficiently edited MYMV and MYMIV genome to provide resistance against cowpea yellow mosaic disease without hampering plant growth and yield.BMC plant biology · 2026Article
- Conquering Limitations: Exploring the Factors that Drive Successful Agrobacterium-Mediated Genetic Transformation of Recalcitrant Plant Species.Molecular biotechnology · 2025Review
- Agrobacterium-mediated genetic transformation of chia (Salvia hispanica L.), a rich source of omega-3 fatty acid.Transgenic research · 2025Article
- Combining the CowPEAsy Web Application With in Planta Agroinfiltration for Native Promoter Validation in Vigna unguiculata.Plant, cell & environment · 2025Article
- Agroinfiltration-mediated transient assay for rapid evaluation of constructs in pigeonpea.Biotechnology notes (Amsterdam, Netherlands) · 2025Article
- Robust soybean leaf agroinfiltration.Plant cell reports · 2024Article
- Overexpression ofHeliyon · 2024Article
- Establishment of an efficient callus transient transformation system for Vitis vinifera cv. 'Chardonnay'.Protoplasma · 2024Article
- Evaluation of Parameters AffectingPlants (Basel, Switzerland) · 2024Article
- CRISPR/Cas9-Mediated Cytosine Base Editing Using an Improved Transformation Procedure in Melon (International journal of molecular sciences · 2023Article
- A simple and efficient protocol for transient transformation of sliced grape berries.Protoplasma · 2023Article
- Recalcitrance to transformation, a hindrance for genome editing of legumes.Frontiers in genome editing · 2023Review
- Ectopic expression ofBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- CRISPR/Cas9 is a powerful tool for precise genome editing of legume crops: a review.Molecular biology reports · 2022Review
- A Method for Developing RNAi-Derived Resistance in Cowpea Against Geminiviruses.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Tissue Culture- and Selection-Independent Agrobacterium tumefaciens-Mediated Transformation of a Recalcitrant Grain Legume, Cowpea (Vigna unguiculata L. Walp).Molecular biotechnology · 2021Article
- Article
- Leaf infiltration in plant science: old method, new possibilities.Plant methods · 2021Review
- Developing a rapid and highly efficient cowpea regeneration, transformation and genome editing system using embryonic axis explants.The Plant journal : for cell and molecular biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An improved method of Agrobacterium-mediated transformation of cowpea was developed employing both sonication and vacuum infiltration treatments. 4 day-old cotyledonary nodes were used as explants for co-cultivation with Agrobacterium tumefaciens strain EHA105 harbouring the binary vector pSouv-cry1Ac. Among the different injury treatments, vacuum infiltration and their combination treatments tested, sonication for 20 s followed by vacuum infiltration for 5 min with A. tumefaciens resulted in highest transient GUS expression efficiency (93% explants expressing GUS at regenerating sites). After 3 days of co-cultivation, the explants were cultured in 150 mg/l kanamycin-containing selection medium and putative transformed plants were recovered. The presence, integration and expression of nptII and cry1Ac genes in T0 transgenic plants were confirmed by polymerase chain reaction (PCR), genomic Southern and qualitative reverse transcription (RT)-PCR analysis. Western blot hybridization and enzyme-linked immunosorbent assay (ELISA) detected and demonstrated the accumulation of Cry1Ac protein in transgenic plants. The cry1Ac gene transmitted in a Mendelian fashion. The stable transformation efficiency increased by 88.4% using both sonication-assisted Agrobacterium-mediated transformation (SAAT) and vacuum infiltration than simple Agrobacterium-mediated transformation in cowpea.
Indexed as
Identifiers
21853337What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.