ArticleTransgenic research2012
High level accumulation of gamma linolenic acid (C18:3Δ6.9,12 cis) in transgenic safflower (Carthamus tinctorius) seeds.
Article in Transgenic research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 71 citations in OpenAlex.
- Recent advances and future directions on GLA-producing organisms.Frontiers in bioengineering and biotechnology · 2025Review
- Two structurally different oomycete lipophilic microbe-associated molecular patterns induce distinctive plant immune responses.Plant physiology · 2024Article
- Synergizing biotechnology and natural farming: pioneering agricultural sustainability through innovative interventions.Frontiers in plant science · 2024Review
- Phylogenetic relationship and sequence diversity of candidate genes involved in anthocyanin biosynthesis pathway inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Engineering and fermenter production of fungi GLA in Pichia pastoris GS115 using oil waste.Archives of microbiology · 2022Article
- A Vernalization Response in a Winter Safflower (Frontiers in plant science · 2021Article
- Endoplasmic reticulum retention signaling and transmembrane channel proteins predicted for oilseed ω3 fatty acid desaturase 3 (FAD3) genes.Functional & integrative genomics · 2020Article
- Producing Cyclopropane Fatty Acid in Plant Leafy BiomassFrontiers in plant science · 2020Article
- High accumulation of γ-linolenic acid and Stearidonic acid in transgenic Perilla (Perilla frutescens var. frutescens) seeds.BMC plant biology · 2019Article
- Seed-specific RNAi in safflower generates a superhigh oleic oil with extended oxidative stability.Plant biotechnology journal · 2018Article
- Functional characterization and substrate specificity analysis of Δ6-desaturase from marine microalga Isochrysis sp.Biotechnology letters · 2018Article
- Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases.Microbial cell factories · 2017Article
- Vegetable Oil: Nutritional and Industrial Perspective.Current genomics · 2016Article
- Stable expression of silencing-suppressor protein enhances the performance and longevity of an engineered metabolic pathway.Plant biotechnology journal · 2016Article
- Fatty Acid Desaturases, Polyunsaturated Fatty Acid Regulation, and Biotechnological Advances.Nutrients · 2016Review
- Reduced Triacylglycerol Mobilization during Seed Germination and Early Seedling Growth in Arabidopsis Containing Nutritionally Important Polyunsaturated Fatty Acids.Frontiers in plant science · 2016Article
- Biosynthesis of Essential Polyunsaturated Fatty Acids in Wheat Triggered by Expression of Artificial Gene.International journal of molecular sciences · 2015Article
- Exploring triacylglycerol biosynthetic pathway in developing seeds of Chia (Salvia hispanica L.): a transcriptomic approach.PloS one · 2015Article
- The effect of evening primrose oil for the prevention of xerotic cheilitis in acne patients being treated with isotretinoin: a pilot study.Annals of dermatology · 2014Article
- Biosynthesis of long chain polyunsaturated fatty acids in the marine ichthyosporean Sphaeroforma arctica.Lipids · 2013Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gamma linolenic acid (GLA; C18:3Δ6,9,12 cis), also known as γ-Linolenic acid, is an important essential fatty acid precursor for the synthesis of very long chain polyunsaturated fatty acids and important pathways involved in human health. GLA is synthesized from linoleic acid (LA; C18:2Δ9,12 cis) by endoplasmic reticulum associated Δ6-desaturase activity. Currently sources of GLA are limited to a small number of plant species with poor agronomic properties, and therefore an economical and abundant commercial source of GLA in an existing crop is highly desirable. To this end, the seed oil of a high LA cultivated species of safflower (Carthamus tinctorius) was modified by transformation with Δ6-desaturase from Saprolegnia diclina resulting in levels exceeding 70% (v/v) of GLA. Levels around 50% (v/v) of GLA in seed oil was achieved when Δ12-/Δ6-desaturases from Mortierella alpina was over-expressed in safflower cultivars with either a high LA or high oleic (OA; C18:1Δ9 cis) background. The differences in the overall levels of GLA suggest the accumulation of the novel fatty acid was not limited by a lack of incorporation into the triacylgylcerol backbone (>66% GLA achieved), or correlated with gene dosage (GLA levels independent of gene copy number), but rather reflected the differences in Δ6-desaturase activity from the two sources. To date, these represent the highest accumulation levels of a newly introduced fatty acid in a transgenic crop. Events from these studies have been propagated and recently received FDA approval for commercialization as Sonova™400.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.