ArticleTransgenic research2012
Mendel: a simple excel workbook to compare the observed and expected distributions of genotypes/phenotypes in transgenic and knockout mouse crosses involving up to three unlinked loci by means of a χ2 test.
Article in Transgenic research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
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- Importing genetically altered animals: ensuring quality.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
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- Breeding of CaScientific reports · 2021Article
- Generation of a conditional Flpo/FRT mouse model expressing constitutively active TGFβ in fibroblasts.Scientific reports · 2020Article
- Interspecific Hybrids BetweenFrontiers in plant science · 2020Article
- CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production.Plant biotechnology journal · 2019Article
- Article
- MCRIP1 promotes the expression of lung-surfactant proteins in mice by disrupting CtBP-mediated epigenetic gene silencing.Communications biology · 2019Article
- Single Nucleotide Polymorphisms in Starch Biosynthetic Genes Associated With Increased Resistant Starch Concentration in Rice Mutant.Frontiers in genetics · 2019Article
- Loss of Mpdz impairs ependymal cell integrity leading to perinatal-onset hydrocephalus in mice.EMBO molecular medicine · 2017Article
- Survival and growth of C57BL/6J mice lacking the BK channel,Physiological reports · 2017Article
- Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR-Cas9-mediated mutagenesis.Nucleic acids research · 2015Article
- NAADP and the two-pore channel protein 1 participate in the acrosome reaction in mammalian spermatozoa.Molecular biology of the cell · 2014Article
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1 author.
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Abstract
The analysis of transgenic and knockout mice always involves the establishment of matings with individuals carrying different loci, segregating independently, whose presence is expected among the progeny, according to a Mendelian distribution. The appearance of distorted inheritance ratios suggests the existence of unexpected lethal or sub-lethal phenotypes associated with some genotypes. These situations are common in a number of cases, including: testing transgenic founder mice for germ-line transmission of their transgenes; setting up heterozygous crosses to obtain homozygous individuals, both for transgenic and knockout mice; establishing matings between floxed mouse lines and suitable cre transgenic mouse lines, etc. The Pearson's χ(2) test can be used to assess the significance of the observed frequencies of genotypes/phenotypes in relation to the expected values, in order to determine whether the observed cases fit the expected distribution. Here, I describe a simple Excel workbook to compare the observed and expected distributions of genotypes/phenotypes in transgenic and knockout mouse crosses involving up to three unlinked loci by means of a χ(2) test. The file is freely available for download from my laboratory's web page at: http://www.cnb.csic.es/~montoliu/Mendel.xls .
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