ArticlePloS one2011
Somaclonal variation is induced de novo via the tissue culture process: a study quantifying mutated cells in Saintpaulia.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Exploring the Significance of Somaclonal Variations in Horticultural Crops.Molecular biotechnology · 2025Review
- Somatic Mutation Accumulations in Micropropagated Cannabis Are Proportional to the Number of Subcultures.Plants (Basel, Switzerland) · 2024Article
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- Comparative restriction enzyme analysis of methylation (CREAM) reveals methylome variability within a clonalFrontiers in plant science · 2024Article
- Optimization of Plant Growth Regulators for In Vitro Mass Propagation of a Disease-Free 'Shine Muscat' Grapevine Cultivar.Current issues in molecular biology · 2023Article
- Cultivar-specific markers, mutations, and chimerisim of Cavendish banana somaclonal variants resistant to Fusarium oxysporum f. sp. cubense tropical race 4.BMC genomics · 2022Article
- Understanding In Vitro Tissue Culture-Induced Variation Phenomenon in Microspore System.International journal of molecular sciences · 2021Review
- Isolating an active and inactive CACTA transposon from lettuce color mutants and characterizing their family.Plant physiology · 2021Article
- RAPD, ISSR, and SCoT markers based genetic stability assessment of micropropagatedPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Medical Cannabis and Industrial Hemp Tissue Culture: Present Status and Future Potential.Frontiers in plant science · 2021Review
- Tissue culture-induced DNA methylation in crop plants: a review.Molecular biology reports · 2021Review
- Cell Wall Epitopes and Endoploidy as Reporters of Embryogenic Potential inInternational journal of molecular sciences · 2018Article
- Review
- SNP-based analysis of genetic diversity in anther-derived rice by whole genome sequencing.Rice (New York, N.Y.) · 2013Article
- A somaclonal line SE7 of finger millet (Eleusine coracana) exhibits modified cytokinin homeostasis and increased grain yield.Journal of experimental botany · 2012Article
- Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications.Plant cell reports · 2012Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe origin of somaclonal variation has not been questioned previously, i.e., "pre-existing mutations" in explants and "newly induced mutations" arising from the tissue culture process have not been distinguished. This is primarily because there has been no reliable molecular method for estimating or quantifying variation. METHODOLOGY/PRINCIPAL
findingsWe adopted a petal-variegated cultivar of Saintpaulia 'Thamires' (Saintpaulia sp.) as the model plant. Based on the difference between the pre- and post-transposon excision sequence of the promoter region of flavonoid 3', 5'-hydoroxylase (F3'5'H), we estimated mutated (transposon-excised) cell percentages using a quantitative real-time PCR. Mutated cell percentages in leaf laminae used as explants was 4.6 and 2.4% in highly or low variegation flower plants, respectively, although the occurrences of blue color mutants in their regenerants were more than 40%. Preexisting mutated cell percentages in cultured explants were considerably lower than the mutated plant percentage among total regenerants via tissue culture. CONCLUSIONS/SIGNIFICANCE: The estimation of mutated cell percentages became possible using the quantitative real-time PCR. The origins of mutations were successfully distinguished; it was confirmed that somaclonal variations are mainly caused by newly generated mutations arising from tissue culture process.
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