ArticlePloS one2011
The microbial detection array combined with random Phi29-amplification used as a diagnostic tool for virus detection in clinical samples.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Characterization of a MERS-related betacoronavirus in Danish brown long-eared bats (Plecotus auritus).Virology journal · 2025Article
- Unbiased Virus Detection in a Danish Zoo Using a Portable Metagenomic Sequencing System.Viruses · 2023Article
- Improvements in metagenomic virus detection by simple pretreatment methods.Journal of clinical virology plus · 2022Article
- Manipulation of the Gut Microbiome Alters Acetaminophen Biodisposition in Mice.Scientific reports · 2020Article
- Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis.PloS one · 2019Article
- Impact of viral presence in tumor on gene expression in non-small cell lung cancer.BMC cancer · 2018Article
- Reply to: Comment on 'Molecular evidence of viral DNA in non-small cell lung cancer and non-neoplastic lung'.British journal of cancer · 2017Article
- Differential expression of viral agents in lymphoma tissues of patients with ABC diffuse large B-cell lymphoma from high and low endemic infectious disease regions.Oncology letters · 2016Article
- Molecular evidence of viral DNA in non-small cell lung cancer and non-neoplastic lung.British journal of cancer · 2016Article
- Investigation of Human Cancers for Retrovirus by Low-Stringency Target Enrichment and High-Throughput Sequencing.Scientific reports · 2015Article
- Target-dependent enrichment of virions determines the reduction of high-throughput sequencing in virus discovery.PloS one · 2015Article
- Ancient pathogen DNA in archaeological samples detected with a Microbial Detection Array.Scientific reports · 2014Article
- Screening of viral pathogens from pediatric ileal tissue samples after vaccination.Advances in virology · 2014Article
- Detection of Epstein-Barr virus (EBV) in human lymphoma tissue by a novel microbial detection array.Biomarker research · 2014Article
- Association of Kaposi’s sarcoma-associated herpesvirus (KSHV) with bladder cancer in Croatian patients.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- The microbial detection array for detection of emerging viruses in clinical samples--a useful panmicrobial diagnostic tool.PloS one · 2014Article
- Article
- Extraneous agent detection in vaccines--a review of technical aspects.Biologicals : journal of the International Association of Biological Standardization · 2012Review
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
A common technique used for sensitive and specific diagnostic virus detection in clinical samples is PCR that can identify one or several viruses in one assay. However, a diagnostic microarray containing probes for all human pathogens could replace hundreds of individual PCR-reactions and remove the need for a clear clinical hypothesis regarding a suspected pathogen. We have established such a diagnostic platform for random amplification and subsequent microarray identification of viral pathogens in clinical samples. We show that Phi29 polymerase-amplification of a diverse set of clinical samples generates enough viral material for successful identification by the Microbial Detection Array, demonstrating the potential of the microarray technique for broad-spectrum pathogen detection. We conclude that this method detects both DNA and RNA virus, present in the same sample, as well as differentiates between different virus subtypes. We propose this assay for diagnostic analysis of viruses in clinical samples.
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