ArticlePloS one2011
DNA profiling of spermatozoa by laser capture microdissection and low volume-PCR.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- A novel 16-plex DIP-SNP panel for forensic analysis of imbalanced DNA mixtures in a Han Chinese population.International journal of legal medicine · 2026Article
- Spitz: a YOLO-based deep learning system for automated spermatozoa detection in forensic genetic analysis.International journal of legal medicine · 2026Article
- Separation and identification of two-individual mixed bloodstains using human neutrophil antigen-1 antibodies.International journal of legal medicine · 2026Article
- Micromanipulation and Multiple-Strand Displacement Amplification for Forensic DNA Profiling of Single Sperm Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Application of a new composite genetic marker semen-specific methylation-microhaplotype in the analysis of semen-vaginal fluid mixtures.Royal Society open science · 2025Article
- Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases.International journal of legal medicine · 2024Review
- Toward Analysis at the Point of Need: A Digital Microfluidic Approach to Processing Multi-Source Sexual Assault Samples.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Single cell genomics applications in forensic science: Current state and future directions.iScience · 2023Review
- Comparison between MACSprep™ forensic sperm microbead kit and Erase Sperm Isolation kit for the enrichment of sperm fractions recovered from sexual assault samples.International journal of legal medicine · 2023Article
- New Perspectives for Whole Genome Amplification in Forensic STR Analysis.International journal of molecular sciences · 2022Review
- Article
- Development of a multiplex methylation-sensitive restriction enzyme-based SNP typing system for deconvolution of semen-containing mixtures.International journal of legal medicine · 2021Article
- Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains.BioMed research international · 2021Article
- Genetic Variants of Microtubule Actin Cross-linking Factor 1 (MACF1) Confer Risk for Parkinson's Disease.Molecular neurobiology · 2017Article
- Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process.International journal of legal medicine · 2017Article
- Isolating cells from female/male blood mixtures using florescence in situ hybridization combined with low volume PCR and its application in forensic science.International journal of legal medicine · 2015Article
- Identification of potential mutations and genomic alterations in the epithelial and spindle cell components of biphasic synovial sarcomas using a human exome SNP chip.BMC medical genomics · 2015Article
- Y-chromosomal haplotyping of single sperm cells isolated from semen mixtures - a successful identification of three perpetrators in a multi-suspect sexual assault case.Croatian medical journal · 2014Article
- Evaluation of parameters in mixed male DNA profiles for the Identifiler® multiplex system.International journal of molecular medicine · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic profiling of sperm from complex biological mixtures such as sexual assault casework samples requires isolation of a pure sperm population and the ability to analyze low abundant samples. Current standard procedure for sperm isolation includes preferential lysis of epithelial contaminants followed by collection of intact sperm by centrifugation. While effective for samples where sperm are abundant, this method is less effective when samples contain few spermatozoa. Laser capture microdissection (LCM) is a proven method for the isolation of cells biological mixtures, even when found in low abundance. Here, we demonstrate the efficacy of LCM coupled with on-chip low volume PCR (LV-PCR) for the isolation and genotyping of low abundance sperm samples. Our results indicate that this method can obtain complete profiles (13-16 loci) from as few as 15 sperm cells with 80% reproducibility, whereas at least 40 sperm cells are required to profile 13-16 loci by standard 'in-tube' PCR. Further, LCM and LV-PCR of a sexual assault casework sample generated a DNA genotype that was consistent with that of the suspect. This method was unable, however, to analyze a casework sample from a gang rape case in which two or more sperm contributors were in a mixed population. The results indicate that LCM and LV-PCR is sensitive and effective for genotyping sperm from sperm/epithelial cell mixtures when epithelial lysis may be insufficient due to low abundance of sperm; LCM and LV-PCR, however, failed in a casework sample when spermatozoa from multiple donors was present, indicating that further study is necessitated.
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