ArticlePLoS genetics2011
Ku must load directly onto the chromosome end in order to mediate its telomeric functions.
Article in PLoS genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Restriction of Ku translocation protects telomere ends.Nature communications · 2025Article
- Telomere length regulation by Rif1 protein fromeLife · 2022Article
- The Multifaceted Roles of Ku70/80.International journal of molecular sciences · 2021Review
- Loss of Ku's DNA end binding activity affects telomere length via destabilizing telomere-bound Est1 rather than altering TLC1 homeostasis.Scientific reports · 2019Article
- KEOPS complex promotes homologous recombination via DNA resection.Nucleic acids research · 2019Article
- ATF7 mediates TNF-α-induced telomere shortening.Nucleic acids research · 2018Article
- Ku DNA End-Binding Activity Promotes Repair Fidelity and Influences End-Processing During Nonhomologous End-Joining inGenetics · 2018Article
- Article
- Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer.The Plant cell · 2017Article
- Acute exercise activates p38 MAPK and increases the expression of telomere-protective genes in cardiac muscle.Experimental physiology · 2017Article
- Functional characterisation of long intergenic non-coding RNAs through genetic interaction profiling in Saccharomyces cerevisiae.BMC biology · 2016Article
- Ku Binding on Telomeres Occurs at Sites Distal from the Physical Chromosome Ends.PLoS genetics · 2016Article
- Consider the workhorse: Nonhomologous end-joining in budding yeast.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2016Review
- Competition between Heterochromatic Loci Allows the Abundance of the Silencing Protein, Sir4, to Regulate de novo Assembly of Heterochromatin.PLoS genetics · 2015Article
- Interplay between Ku and Replication Protein A in the Restriction of Exo1-mediated DNA Break End Resection.The Journal of biological chemistry · 2015Article
- The principal role of Ku in telomere length maintenance is promotion of Est1 association with telomeres.Genetics · 2014Article
- Regulation of Ku-DNA association by Yku70 C-terminal tail and SUMO modification.The Journal of biological chemistry · 2014Article
- Genome-wide studies of telomere biology in budding yeast.Microbial cell (Graz, Austria) · 2014Review
- TRF2 interaction with Ku heterotetramerization interface gives insight into c-NHEJ prevention at human telomeres.Cell reports · 2013Article
- Article
Corrections and comments
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Authors and funding
6 authors.
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Abstract
The Ku heterodimer associates with the Saccharomyces cerevisiae telomere, where it impacts several aspects of telomere structure and function. Although Ku avidly binds DNA ends via a preformed channel, its ability to associate with telomeres via this mechanism could be challenged by factors known to bind directly to the chromosome terminus. This has led to uncertainty as to whether Ku itself binds directly to telomeric ends and whether end association is crucial for Ku's telomeric functions. To address these questions, we constructed DNA end binding-defective Ku heterodimers by altering amino acid residues in Ku70 and Ku80 that were predicted to contact DNA. These mutants continued to associate with their known telomere-related partners, such as Sir4, a factor required for telomeric silencing, and TLC1, the RNA component of telomerase. Despite these interactions, we found that the Ku mutants had markedly reduced association with telomeric chromatin and null-like deficiencies for telomere end protection, length regulation, and silencing functions. In contrast to Ku null strains, the DNA end binding defective Ku mutants resulted in increased, rather than markedly decreased, imprecise end-joining proficiency at an induced double-strand break. This result further supports that it was the specific loss of Ku's telomere end binding that resulted in telomeric defects rather than global loss of Ku's functions. The extensive telomere defects observed in these mutants lead us to propose that Ku is an integral component of the terminal telomeric cap, where it promotes a specific architecture that is central to telomere function and maintenance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.