ArticlePLoS genetics2011
Suppression of scant identifies Endos as a substrate of greatwall kinase and a negative regulator of protein phosphatase 2A in mitosis.
Article in PLoS genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 61 citations in OpenAlex.
- PP2A-Tws dephosphorylates Map205, is required for Polo localization to microtubules and promotes cytokinesis in Drosophila.Cell division · 2024Article
- Greatwall-Endos-PP2A/B55Open biology · 2024Article
- A genetic basis for facultative parthenogenesis in Drosophila.Current biology : CB · 2023Article
- Identification of PP2A-B55 targets uncovers regulation of emerin during nuclear envelope reassembly inOpen biology · 2023Article
- Structural, enzymatic and spatiotemporal regulation of PP2A-B55 phosphatase in the control of mitosis.Frontiers in cell and developmental biology · 2022Review
- Multiple pools of PP2A regulate spindle assembly, kinetochore attachments and cohesion in Drosophila oocytes.Journal of cell science · 2021Article
- Spatiotemporal coordination of Greatwall-Endos-PP2A promotes mitotic progression.The Journal of cell biology · 2021Article
- Greatwall-Endosulfine: A Molecular Switch that Regulates PP2A/B55 Protein Phosphatase Activity in Dividing and Quiescent Cells.International journal of molecular sciences · 2019Review
- Dephosphorylation of Plk1 occurs through PP2A-B55/ENSA/Greatwall pathway during mitotic DNA damage recovery.Cell cycle (Georgetown, Tex.) · 2019Article
- PP2A-B55 promotes nuclear envelope reformation after mitosis inThe Journal of cell biology · 2018Article
- Ensa controls S-phase length by modulating Treslin levels.Nature communications · 2017Article
- The greatwall kinase is dominant over PKA in controlling the antagonistic function of ARPP19 in Xenopus oocytes.Cell cycle (Georgetown, Tex.) · 2017Article
- Article
- Distinct and Overlapping Requirements for Cyclins A, B, and B3 inG3 (Bethesda, Md.) · 2016Article
- A first generation inhibitor of human Greatwall kinase, enabled by structural and functional characterisation of a minimal kinase domain construct.Oncotarget · 2016Article
- A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit.The Journal of cell biology · 2016Article
- Spatial regulation of greatwall by Cdk1 and PP2A-Tws in the cell cycle.Cell cycle (Georgetown, Tex.) · 2016Article
- Entry into mitosis: a solution to the decades-long enigma of MPF.Chromosoma · 2015Review
- The Yeast GSK-3 Homologue Mck1 Is a Key Controller of Quiescence Entry and Chronological Lifespan.PLoS genetics · 2015Article
- Analysis by live imaging of effects of the adenovirus E4orf4 protein on passage through mitosis of H1299 tumor cells.Journal of virology · 2015Article
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10 authors at 3 institutions in 3 countries.
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Abstract
Protein phosphatase 2A (PP2A) plays a major role in dephosphorylating the targets of the major mitotic kinase Cdk1 at mitotic exit, yet how it is regulated in mitotic progression is poorly understood. Here we show that mutations in either the catalytic or regulatory twins/B55 subunit of PP2A act as enhancers of gwl(Scant), a gain-of-function allele of the Greatwall kinase gene that leads to embryonic lethality in Drosophila when the maternal dosage of the mitotic kinase Polo is reduced. We also show that heterozygous mutant endos alleles suppress heterozygous gwl(Scant); many more embryos survive. Furthermore, heterozygous PP2A mutations make females heterozygous for the strong mutation polo(11) partially sterile, even in the absence of gwl(Scant). Heterozygosity for an endos mutation suppresses this PP2A/polo(11) sterility. Homozygous mutation or knockdown of endos leads to phenotypes suggestive of defects in maintaining the mitotic state. In accord with the genetic interactions shown by the gwl(Scant) dominant mutant, the mitotic defects of Endos knockdown in cultured cells can be suppressed by knockdown of either the catalytic or the Twins/B55 regulatory subunits of PP2A but not by the other three regulatory B subunits of Drosophila PP2A. Greatwall phosphorylates Endos at a single site, Ser68, and this is essential for Endos function. Together these interactions suggest that Greatwall and Endos act to promote the inactivation of PP2A-Twins/B55 in Drosophila. We discuss the involvement of Polo kinase in such a regulatory loop.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.