ArticlePLoS genetics2011
Gamma-tubulin is required for bipolar spindle assembly and for proper kinetochore microtubule attachments during prometaphase I in Drosophila oocytes.
Article in PLoS genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 28 citations in OpenAlex.
- Asymmetry of centrosomes inMolecular biology of the cell · 2025Article
- A Brief History ofGenes · 2022Review
- Caught in the Act: Live-Cell Imaging of Plant Meiosis.Frontiers in plant science · 2021Review
- Comparative Cytology of Female Meiosis I AmongG3 (Bethesda, Md.) · 2020Article
- Functional Consequences of the Evolution of Matrimony, a Meiosis-Specific Inhibitor of Polo Kinase.Molecular biology and evolution · 2019Article
- γ-Tubulin⁻γ-Tubulin Interactions as the Basis for the Formation of a Meshwork.International journal of molecular sciences · 2018Review
- Review
- Female Meiosis: Synapsis, Recombination, and Segregation inGenetics · 2018Review
- Article
- Metaphase Spindle Assembly.Biology · 2017Review
- Differentiating the roles of microtubule-associated proteins at meiotic kinetochores during chromosome segregation.Chromosoma · 2016Review
- Oocyte Meiotic Spindle Assembly and Function.Current topics in developmental biology · 2016Review
- The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle.Nature communications · 2015Article
- Normal segregation of a foreign-species chromosome during Drosophila female meiosis despite extensive heterochromatin divergence.Genetics · 2015Article
- Behavior of aberrant chromosome configurations in Drosophila melanogaster female meiosis I.G3 (Bethesda, Md.) · 2014Article
- Topoisomerase II is required for the proper separation of heterochromatic regions during Drosophila melanogaster female meiosis.PLoS genetics · 2014Article
- Article
- Synergy between multiple microtubule-generating pathways confers robustness to centrosome-driven mitotic spindle formation.Developmental cell · 2014Article
- Meiosis-specific stable binding of augmin to acentrosomal spindle poles promotes biased microtubule assembly in oocytes.PLoS genetics · 2013Article
- Binding of Drosophila Polo kinase to its regulator Matrimony is noncanonical and involves two separate functional domains.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many animal species the meiosis I spindle in oocytes is anastral and lacks centrosomes. Previous studies of Drosophila oocytes failed to detect the native form of the germline-specific γ-tubulin (γTub37C) in meiosis I spindles, and genetic studies have yielded conflicting data regarding the role of γTub37C in the formation of bipolar spindles at meiosis I. Our examination of living and fixed oocytes carrying either a null allele or strong missense mutation in the γtub37C gene demonstrates a role for γTub37C in the positioning of the oocyte nucleus during late prophase, as well as in the formation and maintenance of bipolar spindles in Drosophila oocytes. Prometaphase I spindles in γtub37C mutant oocytes showed wide, non-tapered spindle poles and disrupted positioning. Additionally, chromosomes failed to align properly on the spindle and showed morphological defects. The kinetochores failed to properly co-orient and often lacked proper attachments to the microtubule bundles, suggesting that γTub37C is required to stabilize kinetochore microtubule attachments in anastral spindles. Although spindle bipolarity was sometimes achieved by metaphase I in both γtub37C mutants, the resulting chromosome masses displayed highly disrupted chromosome alignment. Therefore, our data conclusively demonstrate a role for γTub37C in both the formation of the anastral meiosis I spindle and in the proper attachment of kinetochore microtubules. Finally, multispectral imaging demonstrates the presences of native γTub37C along the length of wild-type meiosis I spindles.
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