ArticleGenes & development2011
RNAi-independent role for Argonaute2 in CTCF/CP190 chromatin insulator function.
Article in Genes & development, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 130 citations in OpenAlex.
- Review
- Drosophila Rumpelstiltskin regulates Polycomb cis and trans interactions and Homie barrier activity.Epigenetics & chromatin · 2026Article
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- Cooperation between architectural C2H2 proteins in CP190 recruitment to Drosophila regulatory elements.Epigenetics & chromatin · 2025Article
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- MicroRNA: unveiling novel mechanistic and theranostic pathways in diabetic cardiomyopathy.Frontiers in pharmacology · 2025Review
- Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.Nature biotechnology · 2024Article
- New Drosophila promoter-associated architectural protein Mzfp1 interacts with CP190 and is required for housekeeping gene expression and insulator activity.Nucleic acids research · 2024Article
- The N-terminal dimerization domains of human and Drosophila CTCF have similar functionality.Epigenetics & chromatin · 2024Article
- NURF301 contributes to gypsy chromatin insulator-mediated nuclear organization.Nucleic acids research · 2022Article
- Review
- Comparative interactome analysis of the PRE DNA-binding factors: purification of the Combgap-, Zeste-, Psq-, and Adf1-associated proteins.Cellular and molecular life sciences : CMLS · 2022Article
- Argonaute proteins regulate a specific network of genes through KLF4 in mouse embryonic stem cells.Stem cell reports · 2022Article
- Circr, a Computational Tool to Identify miRNA:circRNA Associations.Frontiers in bioinformatics · 2022Article
- Temporal inhibition of chromatin looping and enhancer accessibility during neuronal remodeling.Nature communications · 2021Article
- M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity.Nature communications · 2021Article
- Annotating the Insect Regulatory Genome.Insects · 2021Review
- Nuclear role for human Argonaute-1 as an estrogen-dependent transcription coactivator.The Journal of cell biology · 2020Article
- Article
- Haspin kinase modulates nuclear architecture and Polycomb-dependent gene silencing.PLoS genetics · 2020Article
27 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A major role of the RNAi pathway in Schizosaccharomyces pombe is to nucleate heterochromatin, but it remains unclear whether this mechanism is conserved. To address this question in Drosophila, we performed genome-wide localization of Argonaute2 (AGO2) by chromatin immunoprecipitation (ChIP)-seq in two different embryonic cell lines and found that AGO2 localizes to euchromatin but not heterochromatin. This localization pattern is further supported by immunofluorescence staining of polytene chromosomes and cell lines, and these studies also indicate that a substantial fraction of AGO2 resides in the nucleus. Intriguingly, AGO2 colocalizes extensively with CTCF/CP190 chromatin insulators but not with genomic regions corresponding to endogenous siRNA production. Moreover, AGO2, but not its catalytic activity or Dicer-2, is required for CTCF/CP190-dependent Fab-8 insulator function. AGO2 interacts physically with CTCF and CP190, and depletion of either CTCF or CP190 results in genome-wide loss of AGO2 chromatin association. Finally, mutation of CTCF, CP190, or AGO2 leads to reduction of chromosomal looping interactions, thereby altering gene expression. We propose that RNAi-independent recruitment of AGO2 to chromatin by insulator proteins promotes the definition of transcriptional domains throughout the genome.
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