ArticleThe Journal of biological chemistry2011
Identification and functional and spectral characterization of a globin-coupled histidine kinase from Anaeromyxobacter sp. Fw109-5.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 53 citations in OpenAlex.
- Immunoblot-based activity assay for heme-containing histidine kinases.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2026Article
- The NmpRSTU multi-component signaling system ofJournal of bacteriology · 2025Article
- Expression of hemerythrin-like genes from the obligate aerobeFrontiers in microbiology · 2025Article
- Oxygen-selective regulation of cyclic di-GMP synthesis by a globin coupled sensor with a shortened linking domain modulates Shewanella sp. ANA-3 biofilm.Journal of inorganic biochemistry · 2024Article
- Monitoring the Kinase Activity of Heme-Based Oxygen Sensors and Its Dependence on OMethods in molecular biology (Clifton, N.J.) · 2023Article
- Hydrogen/Deuterium Exchange Mass Spectrometry of Heme-Based Oxygen Sensor Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Characterization of a Cobalt-Substituted Globin-Coupled Oxygen Sensor Histidine Kinase fromACS omega · 2021Article
- Structural modeling of a novel membrane-bound globin-coupled sensor inComputational and structural biotechnology journal · 2021Article
- dbPSP 2.0, an updated database of protein phosphorylation sites in prokaryotes.Scientific data · 2020Article
- Disruption of the dimerization interface of the sensing domain in the dimeric heme-based oxygen sensorThe Journal of biological chemistry · 2020Article
- Suppressor mutations reveal an NtrC-like response regulator, NmpR, for modulation of Type-IV Pili-dependent motility in Myxococcus xanthus.PLoS genetics · 2018Article
- Coordination and redox state-dependent structural changes of the heme-based oxygen sensorThe Journal of biological chemistry · 2017Article
- The importance of conserved amino acids in heme-based globin-coupled diguanylate cyclases.PloS one · 2017Article
- Mechanism and Role of Globin-Coupled Sensor Signalling.Advances in microbial physiology · 2017Review
- An O2-sensing stressosome from a Gram-negative bacterium.Nature communications · 2016Article
- Heme-based globin-coupled oxygen sensors: linking oxygen binding to functional regulation of diguanylate cyclase, histidine kinase, and methyl-accepting chemotaxis.The Journal of biological chemistry · 2013Review
- A heme-based redox sensor in the methanogenic archaeon Methanosarcina acetivorans.The Journal of biological chemistry · 2013Article
- The Heme-Based Oxygen-Sensor Phosphodiesterase Ec DOS (DosP): Structure-Function Relationships.Biosensors · 2013Review
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6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-component signal transduction systems regulate numerous important physiological functions in bacteria. In this study we have identified, cloned, overexpressed, and characterized a dimeric full-length heme-bound (heme:protein, 1:1 stoichiometry) globin-coupled histidine kinase (AfGcHK) from Anaeromyxobacter sp. strain Fw109-5 for the first time. The Fe(III), Fe(II)-O(2), and Fe(II)-CO complexes of the protein displayed autophosphorylation activity, whereas the Fe(II) complex had no significant activity. A H99A mutant lost heme binding ability, suggesting that this residue is the heme proximal ligand. Moreover, His-183 was proposed as the autophosphorylation site based on the finding that the H183A mutant protein was not phosphorylated. The phosphate group of autophosphorylated AfGcHK was transferred to Asp-52 and Asp-169 of a response regulator, as confirmed from site-directed mutagenesis experiments. Based on the amino acid sequences and crystal structures of other globin-coupled oxygen sensor enzymes, Tyr-45 was assumed to be the O(2) binding site at the heme distal side. The O(2) dissociation rate constant, 0.10 s(-1), was substantially increased up to 8.0 s(-1) upon Y45L mutation. The resonance Raman frequencies representing ν(Fe-O2) (559 cm(-1)) and ν(O-O) (1149 cm(-1)) of the Fe(II)-O(2) complex of Y45F mutant AfGcHK were distinct from those of the wild-type protein (ν(Fe-O2), 557 cm(-1); ν(O-O), 1141 cm(-1)), supporting the proposal that Tyr-45 is located at the distal side and forms hydrogen bonds with the oxygen molecule bound to the Fe(II) complex. Thus, we have successfully identified and characterized a novel heme-based globin-coupled oxygen sensor histidine kinase, AfGcHK, in this study.
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