ArticleACS nano2011
High content screening in zebrafish speeds up hazard ranking of transition metal oxide nanoparticles.
Article in ACS nano, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 201 citations in OpenAlex.
- Nano-engineering of a thermoplastic compound with CuO nanoparticles for the development of safe antimicrobial and anti-biofouling fish cage nets.RSC advances · 2025Article
- Comparative toxicity and environmental impact assessments of sonochemically-synthesized CuO and Zn-doped CuO nanoparticles using zebrafish and LCA tools.Discover nano · 2025Article
- Utilization of Artificial Intelligence Coupled with a High-Throughput, High-Content Platform in the Exploration of Neurodevelopmental Toxicity of Individual and Combined PFAS.Journal of xenobiotics · 2025Article
- Chemical screens for particle-induced macrophage death identifies kinase inhibitors of phagocytosis as targets for toxicity.Journal of nanobiotechnology · 2024Article
- Remediation of Metal Oxide Nanotoxicity with a Functional Amyloid.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Thallium's Threat to Aquatic Life: Stage-Specific Toxicity in Zebrafish Embryos and Larvae.Environment & health (Washington, D.C.) · 2024Article
- Zebrafish Insights into Nanomaterial Toxicity: A Focused Exploration on Metallic, Metal Oxide, Semiconductor, and Mixed-Metal Nanoparticles.International journal of molecular sciences · 2024Review
- Toxicity of Titanium Dioxide-Cerium Oxide Nanocomposites to Zebrafish Embryos: A Preliminary Evaluation.Toxics · 2023Article
- Titanium Dioxide Nanoparticles: Effects on Development and Male Reproductive System.Nanomaterials (Basel, Switzerland) · 2023Article
- High-throughput screening of nanoparticles in drug delivery.APL bioengineering · 2021Review
- Zebrafish: A Promising Real-Time Model System for Nanotechnology-Mediated Neurospecific Drug Delivery.Nanoscale research letters · 2021Review
- Article
- Toxicity of Nanoparticulate Nickel to Aquatic Organisms: Review and Recommendations for Improvement of Toxicity Tests.Environmental toxicology and chemistry · 2020Review
- Safety Considerations of Cancer Nanomedicine-A Key Step toward Translation.Small (Weinheim an der Bergstrasse, Germany) · 2020Review
- Assessment of Cu and CuO nanoparticle ecological responses using laboratory small-scale microcosms.Environmental science. Nano · 2020Article
- Inhibition of amyloid beta toxicity in zebrafish with a chaperone-gold nanoparticle dual strategy.Nature communications · 2019Article
- A Comparative Assessment of Nanotoxicity Induced by Metal (Silver, Nickel) and Metal Oxide (Cobalt, Chromium) Nanoparticles inNanomaterials (Basel, Switzerland) · 2019Article
- Physical and Toxicological Profiles of Human IAPP Amyloids and Plaques.Science bulletin · 2019Article
- Mitigating Human IAPP Amyloidogenesis In Vivo with Chiral Silica Nanoribbons.Small (Weinheim an der Bergstrasse, Germany) · 2018Article
- Zebrafish as a Model to Evaluate Nanoparticle Toxicity.Nanomaterials (Basel, Switzerland) · 2018Review
Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
Abstract
Zebrafish is an aquatic organism that can be used for high content safety screening of engineered nanomaterials (ENMs). We demonstrate, for the first time, the use of high content bright-field and fluorescence-based imaging to compare the toxicological effect of transition metal oxide (CuO, ZnO, NiO, and Co(3)O(4)) nanoparticles in zebrafish embryos and larvae. High content bright-field imaging demonstrated potent and dose-dependent hatching interference in the embryos, with the exception of Co(3)O(4) which was relatively inert. We propose that the hatching interference was due to the shedding of Cu and Ni ions, compromising the activity of the hatching enzyme, ZHE1, similar to what we previously proposed for Zn(2+). This hypothesis is based on the presence of metal-sensitive histidines in the catalytic center of this enzyme. Co-introduction of a metal ion chelator, diethylene triamine pentaacetic acid (DTPA), reversed the hatching interference of Cu, Zn, and Ni. While neither the embryos nor larvae demonstrated morphological abnormalities, high content fluorescence-based imaging demonstrated that CuO, ZnO, and NiO could induce increased expression of the heat shock protein 70:enhanced green fluorescence protein (hsp70:eGFP) in transgenic zebrafish larvae. Induction of this response by CuO required a higher nanoparticle dose than the amount leading to hatching interference. This response was also DTPA-sensitive. We demonstrate that high content imaging of embryo development, morphological abnormalities, and HSP70 expression can be used for hazard ranking and determining the dose-response relationships leading to ENM effects on the development of the zebrafish embryo.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.