ArticlePloS one2011
Gas6 downregulation impaired cytoplasmic maturation and pronuclear formation independent to the MPF activity.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- Maternal effect factors that contribute to oocytes developmental competence: an update.Journal of assisted reproduction and genetics · 2022Review
- Article
- Gas6 is a reciprocal regulator of mitophagy during mammalian oocyte maturation.Scientific reports · 2019Article
- Genomic insights into chromatin reprogramming to totipotency in embryos.The Journal of cell biology · 2019Review
- Dynamics of the epigenetic landscape during the maternal-to-zygotic transition.Nature reviews. Molecular cell biology · 2018Review
- The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development.Open biology · 2016Article
- The role of Rad51 in safeguarding mitochondrial activity during the meiotic cell cycle in mammalian oocytes.Scientific reports · 2016Article
- The Role of Maternal-Effect Genes in Mammalian Development: Are Mammalian Embryos Really an Exception?Stem cell reviews and reports · 2016Review
- Effect of L-carnitine supplementation on maturation and early embryo development of immature mouse oocytes selected by brilliant cresyle blue staining.Journal of assisted reproduction and genetics · 2015Article
- Article
- Maternal effect genes: Findings and effects on mouse embryo development.Clinical and experimental reproductive medicine · 2014Review
- Molecular determinants of the meiotic arrests in mammalian oocytes at different stages of maturation.Cell cycle (Georgetown, Tex.)Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we found that the growth arrest-specific gene 6 (Gas6) is more highly expressed in germinal vesicle (GV) oocytes than in metaphase II (MII) oocytes using annealing control primer (ACP)-PCR technology. The current study was undertaken to investigate the role of Gas6 in oocyte maturation and fertilization using RNA interference (RNAi). Interestingly, despite the specific and marked decrease in Gas6 mRNA and protein expression in GVs after Gas6 RNAi, nuclear maturation including spindle structures and chromosome segregation was not affected. The only discernible effect induced by Gas6 RNAi was a change in maturation promoting factor (MPF) activity. After parthenogenetic activation, Gas6 RNAi-treated oocytes at the MII stage had not developed further and arrested at MII (90.0%). After stimulation with Sr(2+), Gas6-silenced MII oocytes had markedly reduced Ca(2+) oscillation and exhibited no exocytosis of cortical granules. In these oocytes, sperm penetration occurred during fertilization but not pronucleus (PN) formation. By roscovitine and colcemid treatment, we found that the Gas6 knockdown affected cytoplasmic maturation directly, independent to the changed MPF activity. These results strongly suggest that 1) the Gas6 signaling itself is important to the cytoplasmic maturation, but not nuclear maturation, and 2) the decreased Gas6 expression and decreased MPF activity separately or mutually influence sperm head decondensation and PN formation.
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