ArticlePloS one2011
Microencapsulation technology: a powerful tool for integrating expansion and cryopreservation of human embryonic stem cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Multiscale design of core-shell GelMA-alginate composite microspheres enabling spatially compartmentalized co-culture of skin cells.Materials today. Bio · 2026Article
- Biological Models for Evaluating Hydrogel-Based Formulations in Wound Healing.Gels (Basel, Switzerland) · 2025Review
- Challenges and Considerations of Preclinical Development for iPSC-Based Myogenic Cell Therapy.Cells · 2024Review
- Fabricating 3-dimensional human brown adipose microtissues for transplantation studies.Bioactive materials · 2023Article
- Engineered T cells from induced pluripotent stem cells: from research towards clinical implementation.Frontiers in immunology · 2023Review
- Review
- Article
- Development of Serum-Free Media for Cryopreservation of Hydrogel Encapsulated Cell-Based Therapeutics.Cellular and molecular bioengineering · 2022Article
- Cytoprotection of Human Progenitor and Stem Cells through Encapsulation in Alginate Templated, Dual Crosslinked Silk and Silk-Gelatin Composite Hydrogel Microbeads.Advanced healthcare materials · 2022Article
- Manufacturing clinical-grade human induced pluripotent stem cell-derived beta cells for diabetes treatment.Cell proliferation · 2022Review
- Patient-Derived Breast Cancer Tissue Cultures for Anti-Endocrine Drug Assays.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coating.Frontiers in chemical engineering · 2022Article
- Preparation of Alginate-Based Biomaterials and Their Applications in Biomedicine.Marine drugs · 2021Review
- Coaxial Alginate Hydrogels: From Self-Assembled 3D Cellular Constructs to Long-Term Storage.International journal of molecular sciences · 2021Article
- Transcriptomic Analysis of Naïve Human Embryonic Stem Cells Cultured in Three-Dimensional PEG Scaffolds.Biomolecules · 2020Article
- MSC encapsulation in alginate microcapsules prolongs survival after intra-articular injection, a longitudinal in vivo cell and bead integrity tracking study.Cell biology and toxicology · 2020Article
- Toward a Microencapsulated 3D hiPSC-DerivedFrontiers in bioengineering and biotechnology · 2020Article
- Cells, Materials, and Fabrication Processes for Cardiac Tissue Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- Three dimensional microcarrier system in mesenchymal stem cell culture: a systematic review.Cell & bioscience · 2020Review
- Developments in cell culture systems for human pluripotent stem cells.World journal of stem cells · 2019Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The successful implementation of human embryonic stem cells (hESCs)-based technologies requires the production of relevant numbers of well-characterized cells and their efficient long-term storage. In this study, cells were microencapsulated in alginate to develop an integrated bioprocess for expansion and cryopreservation of pluripotent hESCs. Different three-dimensional (3D) culture strategies were evaluated and compared, specifically, microencapsulation of hESCs as: i) single cells, ii) aggregates and iii) immobilized on microcarriers. In order to establish a scalable bioprocess, hESC-microcapsules were cultured in stirred tank bioreactors.The combination of microencapsulation and microcarrier technology resulted in a highly efficient protocol for the production and storage of pluripotent hESCs. This strategy ensured high expansion ratios (an approximately twenty-fold increase in cell concentration) and high cell recovery yields (>70%) after cryopreservation. When compared with non-encapsulated cells, cell survival post-thawing demonstrated a three-fold improvement without compromising hESC characteristics.Microencapsulation also improved the culture of hESC aggregates by protecting cells from hydrodynamic shear stress, controlling aggregate size and maintaining cell pluripotency for two weeks.This work establishes that microencapsulation technology may prove a powerful tool for integrating the expansion and cryopreservation of pluripotent hESCs. The 3D culture strategy developed herein represents a significant breakthrough towards the implementation of hESCs in clinical and industrial applications.
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