ArticlePloS one2011
Actin fusion proteins alter the dynamics of mechanically induced cytoskeleton rearrangement.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- Review
- Hypergravity Attenuates Reactivity in Primary Murine Astrocytes.Biomedicines · 2022Article
- Actomyosin contractility and buckling of microtubules in nucleation, growth and disassembling of focal adhesions.Biomechanics and modeling in mechanobiology · 2022Article
- A Fully Integrated Arduino-Based System for the Application of Stretching Stimuli to Living Cells and Their Time-Lapse Observation: A Do-It-Yourself Biology Approach.Annals of biomedical engineering · 2021Article
- Super-Resolution Imaging Approaches for Quantifying F-Actin in Immune Cells.Frontiers in cell and developmental biology · 2021Review
- Interrogating Synaptic Architecture: Approaches for Labeling Organelles and Cytoskeleton Components.Frontiers in synaptic neuroscience · 2019Review
- Hydrodynamics in Cell Studies.Chemical reviews · 2018Review
- Article
- The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Nanoscopic imaging of thick heterogeneous soft-matter structures in aqueous solution.Nature communications · 2016Article
- Differential effects of LifeAct-GFP and actin-GFP on cell mechanics assessed using micropipette aspiration.Journal of biomechanics · 2016Article
- Heading in the Right Direction: Understanding Cellular Orientation Responses to Complex Biophysical Environments.Cellular and molecular bioengineering · 2016Review
- Temporal responses of human endothelial and smooth muscle cells exposed to uniaxial cyclic tensile strain.Experimental biology and medicine (Maywood, N.J.) · 2015Article
- In vivo imaging of Nematostella vectensis embryogenesis and late development using fluorescent probes.BMC cell biology · 2014Article
- The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis.Developmental biology · 2014Article
- Tissue-specific mechanical and geometrical control of cell viability and actin cytoskeleton alignment.Scientific reports · 2014Article
- New single-molecule speckle microscopy reveals modification of the retrograde actin flow by focal adhesions at nanometer scales.Molecular biology of the cell · 2014Article
- Measuring F-actin properties in dendritic spines.Frontiers in neuroanatomy · 2014Article
- Endothelial cell-cell adhesion during zebrafish vascular development.Cell adhesion & migration · 2014Review
- Measuring actin flow in 3D cell protrusions.Biophysical journal · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanical forces can regulate various functions in living cells. The cytoskeleton is a crucial element for the transduction of forces in cell-internal signals and subsequent biological responses. Accordingly, many studies in cellular biomechanics have been focused on the role of the contractile acto-myosin system in such processes. A widely used method to observe the dynamic actin network in living cells is the transgenic expression of fluorescent proteins fused to actin. However, adverse effects of GFP-actin fusion proteins on cell spreading, migration and cell adhesion strength have been reported. These shortcomings were shown to be partly overcome by fusions of actin binding peptides to fluorescent proteins. Nevertheless, it is not understood whether direct labeling by actin fusion proteins or indirect labeling via these chimaeras alters biomechanical responses of cells and the cytoskeleton to forces. We investigated the dynamic reorganization of actin stress fibers in cells under cyclic mechanical loading by transiently expressing either egfp-Lifeact or eyfp-actin in rat embryonic fibroblasts and observing them by means of live cell microscopy. Our results demonstrate that mechanically-induced actin stress fiber reorganization exhibits very different kinetics in EYFP-actin cells and EGFP-Lifeact cells, the latter showing a remarkable agreement with the reorganization kinetics of non-transfected cells under the same experimental conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.