ArticleMolecular biology of the cell2011
Rab22 controls NGF signaling and neurite outgrowth in PC12 cells.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Pooled it
- Vesicular transport in the autophagic route: RAB GTPases as pivotal regulators of autophagy.The Biochemical journal · 2025Review
- Rab22a regulates the establishment of epithelial polarity.Small GTPases · 2021Article
- Use of Immunohistochemistry to Determine Expression of Rab5 Subfamily of GTPases in Mature and Developmental Brains.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Dysregulated Plasma Membrane Turnover Underlying Dendritic Pathology in Neurodegenerative Diseases.Frontiers in cellular neuroscience · 2020Review
- Coordination of AMPA receptor trafficking by Rab GTPases.Small GTPases · 2019Review
- Optogenetic Delineation of Receptor Tyrosine Kinase Subcircuits in PC12 Cell Differentiation.Cell chemical biology · 2019Article
- Consequences of Rab GTPase dysfunction in genetic or acquired human diseases.Small GTPases · 2018Review
- Bioactivity-Guided Isolation of Neuritogenic Factor from the Seeds of the Gac Plant (Evidence-based complementary and alternative medicine : eCAM · 2018Article
- Arf6 and Rab22 mediate T cell conjugate formation by regulating clathrin-independent endosomal membrane trafficking.Journal of cell science · 2017Article
- Neurotrophic Effects of Mu Bie Zi (Momordica cochinchinensis) Seed Elucidated by High-Throughput Screening of Natural Products for NGF Mimetic Effects in PC-12 Cells.Neurochemical research · 2015Article
- Role of Rab GTPases and their interacting proteins in mediating metabolic signalling and regulation.Cellular and molecular life sciences : CMLS · 2015Review
- Cortex Mori Radicis Extract induces neurite outgrowth in PC12 cells activating ERK signaling pathway via inhibiting Ca(2+) influx.International journal of clinical and experimental medicine · 2015Article
- Biogenesis and function of the NGF/TrkA signaling endosome.International review of cell and molecular biology · 2015Review
- Differential effects of overexpression of Rab5 and Rab22 on autophagy in PC12 cells with or without NGF.Methods in molecular biology (Clifton, N.J.) · 2015Article
- RabGEF1/Rabex-5 Regulates TrkA-Mediated Neurite Outgrowth and NMDA-Induced Signaling Activation in NGF-Differentiated PC12 Cells.PloS one · 2015Article
- Distinct biochemical and functional properties of two Rab5 homologs from the rice blast fungus Magnaporthe oryzae.The Journal of biological chemistry · 2014Article
- The role of rab proteins in neuronal cells and in the trafficking of neurotrophin receptors.Membranes · 2014Review
- Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis.Journal of cell science · 2014Article
- Light-mediated kinetic control reveals the temporal effect of the Raf/MEK/ERK pathway in PC12 cell neurite outgrowth.PloS one · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Rab22 is a small GTPase that is localized on early endosomes and regulates early endosomal sorting. This study reports that Rab22 promotes nerve growth factor (NGF) signaling-dependent neurite outgrowth and gene expression in PC12 cells by sorting NGF and the activated/phosphorylated receptor (pTrkA) into signaling endosomes to sustain signal transduction in the cell. NGF binding induces the endocytosis of pTrkA into Rab22-containing endosomes. Knockdown of Rab22 via small hairpin RNA (shRNA) blocks NGF-induced pTrkA endocytosis into the endosomes and gene expression (VGF) and neurite outgrowth. Overexpression of human Rab22 can rescue the inhibitory effects of the Rab22 shRNA, suggesting a specific Rab22 function in NGF signal transduction, rather than off-target effects. Furthermore, the Rab22 effector, Rabex-5, is necessary for NGF-induced neurite outgrowth and gene expression, as evidenced by the inhibitory effect of shRNA-mediated knockdown of Rabex-5. Disruption of the Rab22-Rabex-5 interaction via overexpression of the Rab22-binding domain of Rabex-5 in the cell also blocks NGF-induced neurite outgrowth, suggesting a critical role of Rab22-Rabex-5 interaction in the biogenesis of NGF-signaling endosomes to sustain the signal for neurite outgrowth. These data provide the first evidence for an early endosomal Rab GTPase as a positive regulator of NGF signal transduction and cell differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.