ArticleMolecular plant pathology2012
Pectate lyase affects pathogenicity in natural isolates of Colletotrichum coccodes and in pelA gene-disrupted and gene-overexpressing mutant lines.
Article in Molecular plant pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 61 citations in OpenAlex.
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- Dual activation of soybean resistance against Phytophthora sojae by pectin lyase and degraded pectin oligosaccharides.Science China. Life sciences · 2024Article
- Dual Transcriptome Analysis Reveals the Changes in Gene Expression in Both Cotton andJournal of fungi (Basel, Switzerland) · 2024Article
- Effects of Ambient pH on the Growth and Development, Pathogenicity, and Diacetoxyscirpenol Accumulation of Muskmelon Fruit Caused byJournal of fungi (Basel, Switzerland) · 2024Article
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- Genomic insights into bamboo witches' broom disease: pathogenicity and phytohormone biosynthesis inFrontiers in microbiology · 2024Article
- The glycoside hydrolase 28 member VdEPG1 is a virulence factor of Verticillium dahliae and interacts with the jasmonic acid pathway-related gene GhOPR9.Molecular plant pathology · 2023Article
- VmPacC-mediated pH regulation of Valsa mali confers to host acidification identified by comparative proteomics analysis.Stress biology · 2023Article
- Pectate Lyase from Fusarium sacchari Induces Plant Immune Responses and Contributes to Virulence.Microbiology spectrum · 2023Article
- Genomic and Transcriptomic Survey Provides Insights into Molecular Basis of Pathogenicity of the Sunflower PathogenJournal of fungi (Basel, Switzerland) · 2023Article
- Pectate Lyase Genes Abundantly Expressed During the Infection Regulate Morphological Development ofmSphere · 2023Article
- Transcription Factor VdCf2 Regulates Growth, Pathogenicity, and the Expression of a Putative Secondary Metabolism Gene Cluster in Verticillium dahliae.Applied and environmental microbiology · 2022Article
- Selection of potential reference genes for RT-qPCR in the plant pathogenic fungusFrontiers in microbiology · 2022Article
- Genome Analysis of the Broad Host Range NecrotrophFrontiers in plant science · 2022Article
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3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Colletotrichum coccodes (Wallr.) S. Hughes, the causal agent of black dot on potato and anthracnose on tomato, reduces yield and crop quality. We explored the role of secreted pectate lyase (PL), a cell wall-degrading enzyme, in the aggressiveness of C. coccodes. In vitro-cultivated highly aggressive isolates secreted immunologically detectable PL levels 6 h after transfer to secondary medium versus 12 h for mildly aggressive isolates, suggesting that secreted PL is a virulence factor. The gene encoding PL, CcpelA, was cloned and used for the genetic manipulation of highly (US-41 and Si-72) and mildly (Si-60) aggressive isolates. CcpelA gene-disrupted mutants showed reduced aggressiveness towards tomato fruits and impaired PL secretion and extracellular activity. Conversely, overexpression of CcpelA in the Si-60 isolate increased its aggressiveness and PL secretion. Comparison of CcpelA cloned from isolates US-41 and Si-60 revealed that both encode identical proteins, but differ in their promoters. Bioinformatics analysis for cis-acting elements suggested that the promoters of the US-41 and Si-60 isolates contain one and no AreA-binding site (GATA box), respectively. AreA has been suggested to be involved in fungal aggressiveness; therefore, CcpelA may be a key virulence factor in C. coccodes pathogenicity, and the differences in isolate aggressiveness might result from promoter activity. Quantitative reverse transcriptase-polymerase chain reaction analyses confirmed the higher level of CcpelA transcript in isolate US-41 versus Si-60.
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