ArticleAmerican journal of physiology. Endocrinology and metabolism2011
The phospholipid monolayer associated with perilipin-enriched lipid droplets is a highly organized rigid membrane structure.
Article in American journal of physiology. Endocrinology and metabolism, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Hydrophilic polymeric fluorescent probes based on benzothiadiazole constructed for real-time monitoring of lipid droplet levels in cells.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
- Traumatic brain injury reprograms lipid droplet metabolism shaped by aging and diet in Drosophila brain.PloS one · 2025Article
- Article
- Perilipins at a glance.Journal of cell science · 2022Article
- The C-Terminus of Perilipin 3 Shows Distinct Lipid Binding at Phospholipid-Oil-Aqueous Interfaces.Membranes · 2021Article
- Energy and Dynamics of Caveolae Trafficking.Frontiers in cell and developmental biology · 2020Review
- Phosphorylation of PLIN3 by AMPK promotes dispersion of lipid droplets during starvation.Protein & cell · 2019Article
- Sizing lipid droplets from adult and geriatric mouse liver tissue via nanoparticle tracking analysis.Analytical and bioanalytical chemistry · 2018Article
- ATGL Promotes Autophagy/Lipophagy via SIRT1 to Control Hepatic Lipid Droplet Catabolism.Cell reports · 2017Article
- Clues on the function of Manduca sexta perilipin 2 inferred from developmental and nutrition-dependent changes in its expression.Insect biochemistry and molecular biology · 2017Article
- ATP Binding Cassette Transporter ABCA7 Regulates NKT Cell Development and Function by Controlling CD1d Expression and Lipid Raft Content.Scientific reports · 2017Article
- Insertion of perilipin 3 into a glycero(phospho)lipid monolayer depends on lipid headgroup and acyl chain species.Journal of lipid research · 2016Article
- Synaptogyrin-2 Promotes Replication of a Novel Tick-borne Bunyavirus through Interacting with Viral Nonstructural Protein NSs.The Journal of biological chemistry · 2016Article
- Article
- Fatty acid signaling: the new function of intracellular lipases.International journal of molecular sciences · 2015Review
- Comparative analysis of lipotoxicity induced by endocrine, pharmacological, and innate immune stimuli in rat basophilic leukemia cells.Journal of immunotoxicology · 2015Article
- GRAF1a is a brain-specific protein that promotes lipid droplet clustering and growth, and is enriched at lipid droplet junctions.Journal of cell science · 2014Article
- Surface features of the lipid droplet mediate perilipin 2 localization.Biochemical and biophysical research communications · 2014Article
- Role of adipose specific lipid droplet proteins in maintaining whole body energy homeostasis.Biochimica et biophysica acta · 2014Review
- Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice.Nutrition & diabetes · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The significance of lipid droplets (LD) in lipid metabolism, cell signaling, and membrane trafficking is increasingly recognized, yet the role of the LD phospholipid monolayer in LD protein targeting and function remains unknown. To begin to address this issue, two populations of LD were isolated by ConA sepharose affinity chromatography: 1) functionally active LD enriched in perilipin, caveolin-1, and several lipolytic proteins, including ATGL and HSL; and 2) LD enriched in ADRP and TIP47 that contained little to no lipase activity. Coimmunoprecipitation experiments confirmed the close association of caveolin and perilipin and lack of interaction between caveolin and ADRP, in keeping with the separation observed with the ConA procedure. The phospholipid monolayer structure was evaluated to reveal that the perilipin-enriched LD exhibited increased rigidity (less fluidity), as shown by increased cholesterol/phospholipid, Sat/Unsat, and Sat/MUFA ratios. These results were confirmed by DPH-TMA, NBD-cholesterol, and NBD-sphingomyelin fluorescence polarization studies. By structure and organization, the perilipin-enriched LD most closely resembled the adipocyte PM. In contrast, the ADRP/TIP47-enriched LD contained a more fluid monolayer membrane, reflecting decreased polarizations and lipid order based on phospholipid fatty acid analysis. Taken together, results indicate that perilipin and associated lipolytic enzymes target areas in the phospholipid monolayer that are highly organized and rigid, similar in structure to localized areas of the PM where cholesterol and fatty acid uptake and efflux occur.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.